Poly(beta-amino esters): procedures for synthesis and gene delivery.

Jordan J. Green, Gregory T. Zugates, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticlepeer-review

Abstract

Non-viral gene delivery systems are promising as they avoid many problems of viral gene therapy by having increased design flexibility, high safety, large DNA cargo capacity, and ease of manufacture. Here, we describe the use of polymeric vectors, in particular biodegradable poly(beta-amino esters) (PBAEs), for non-viral gene delivery. These polymers are able to self-assemble with DNA and form positively charged gene delivery nanoparticles. Methods for synthesis of these polymers, particle self-assembly, and transfection using these particles are delineated. A standard protocol is presented as well as a high-throughput screening technique that can be used to more quickly optimize transfection parameters for efficient delivery.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume480
StatePublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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