Polyamines in rat brain extracellular space after ischemia

Gad M. Gilad; Robert A. Casero; Raul Busto; Mordecai Y.T. Globus

doi:10.1007/BF03160020

Polyamines in rat brain extracellular space after ischemia

Gad M. Gilad, Robert A. Casero, Raul Busto, Mordecai Y.T. Globus

School of Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

This article reports measurements of extracellular polyamines in rat brain. Microdialysis probes were implanted in the striatum and microperfusion samples were collected before, during, and after transient (20 min) global ischemia. Polyamine levels in microperfusates were measured by reverse-phase high-performance liquid chromatography after precolumn derivatization. No significant changes in extracellular polyamines were detected for up to 3 h following ischemia. We conclude that extracellular levels of polyamines (1) are comparable to the low levels in other extracellular compartments, and (2) do not change during the initial period of reperfusion, the period most critical for neuron survival after ischemia.

Original language	English (US)
Pages (from-to)	27-33
Number of pages	7
Journal	Molecular and Chemical Neuropathology
Volume	18
Issue number	1-2
DOIs	https://doi.org/10.1007/BF03160020
State	Published - Jan 1993

Keywords

Polyamines, extracellular putrescine, spermidine and spermine
brain, dorsolateral region of the rat corpus striatum
high performance liquid chromatography (HPLC), polyamine measurements by HPLC
ischemia, transient global ischemia
microdialysis, extracellular fluid collection by microdialysis probe

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology

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10.1007/BF03160020

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title = "Polyamines in rat brain extracellular space after ischemia",

abstract = "This article reports measurements of extracellular polyamines in rat brain. Microdialysis probes were implanted in the striatum and microperfusion samples were collected before, during, and after transient (20 min) global ischemia. Polyamine levels in microperfusates were measured by reverse-phase high-performance liquid chromatography after precolumn derivatization. No significant changes in extracellular polyamines were detected for up to 3 h following ischemia. We conclude that extracellular levels of polyamines (1) are comparable to the low levels in other extracellular compartments, and (2) do not change during the initial period of reperfusion, the period most critical for neuron survival after ischemia.",

keywords = "Polyamines, extracellular putrescine, spermidine and spermine, brain, dorsolateral region of the rat corpus striatum, high performance liquid chromatography (HPLC), polyamine measurements by HPLC, ischemia, transient global ischemia, microdialysis, extracellular fluid collection by microdialysis probe",

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AU - Casero, Robert A.

AU - Busto, Raul

AU - Globus, Mordecai Y.T.

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N2 - This article reports measurements of extracellular polyamines in rat brain. Microdialysis probes were implanted in the striatum and microperfusion samples were collected before, during, and after transient (20 min) global ischemia. Polyamine levels in microperfusates were measured by reverse-phase high-performance liquid chromatography after precolumn derivatization. No significant changes in extracellular polyamines were detected for up to 3 h following ischemia. We conclude that extracellular levels of polyamines (1) are comparable to the low levels in other extracellular compartments, and (2) do not change during the initial period of reperfusion, the period most critical for neuron survival after ischemia.

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Polyamines in rat brain extracellular space after ischemia

Abstract

Keywords

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