Polyamines in rat brain extracellular space after ischemia

Gad M. Gilad, Robert A. Casero, Raul Busto, Mordecai Y.T. Globus

Research output: Contribution to journalArticlepeer-review


This article reports measurements of extracellular polyamines in rat brain. Microdialysis probes were implanted in the striatum and microperfusion samples were collected before, during, and after transient (20 min) global ischemia. Polyamine levels in microperfusates were measured by reverse-phase high-performance liquid chromatography after precolumn derivatization. No significant changes in extracellular polyamines were detected for up to 3 h following ischemia. We conclude that extracellular levels of polyamines (1) are comparable to the low levels in other extracellular compartments, and (2) do not change during the initial period of reperfusion, the period most critical for neuron survival after ischemia.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalMolecular and Chemical Neuropathology
Issue number1-2
StatePublished - Jan 1993


  • Polyamines, extracellular putrescine, spermidine and spermine
  • brain, dorsolateral region of the rat corpus striatum
  • high performance liquid chromatography (HPLC), polyamine measurements by HPLC
  • ischemia, transient global ischemia
  • microdialysis, extracellular fluid collection by microdialysis probe

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology


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