Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation

Wenqiang Yu, Vasudeva Ginjala, Vinod Pant, Igor Chernukhin, Joanne Whitehead, France Docquier, Dawn Farrar, Gholamreza Tavoosidana, Rituparna Mukhopadhyay, Chandrasekhar Kanduri, Mitsuo Oshimura, Andrew P. Feinberg, Victor Lobanenkov, Elena Klenova, Rolf Ohlsson

Research output: Contribution to journalArticle

Abstract

Chromatin insulators demarcate expression domains by blocking the cis effects of enhancers or silencers in a position-dependent manner. We show that the chromatin insulator protein CTCF carries a post-translational modification: poly(ADP-ribosyl)ation. Chromatin immunoprecipitation analysis showed that a poly(ADP-ribosyl)ation mark, which exclusively segregates with the maternal allele of the insulator domain in the H19 imprinting control region, requires the bases that are essential for interaction with CTCF. Chromatin immunoprecipitation-on-chip analysis documented that the link between CTCF and poly(ADP-ribosyI)ation extended to more than 140 mouse CTCF target sites. An insulator trap assay showed that the insulator function of most of these CTCF target sites is sensitive to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase activity. We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions.

Original languageEnglish (US)
Pages (from-to)1105-1110
Number of pages6
JournalNature genetics
Volume36
Issue number10
DOIs
StatePublished - Oct 1 2004

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation'. Together they form a unique fingerprint.

  • Cite this

    Yu, W., Ginjala, V., Pant, V., Chernukhin, I., Whitehead, J., Docquier, F., Farrar, D., Tavoosidana, G., Mukhopadhyay, R., Kanduri, C., Oshimura, M., Feinberg, A. P., Lobanenkov, V., Klenova, E., & Ohlsson, R. (2004). Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation. Nature genetics, 36(10), 1105-1110. https://doi.org/10.1038/ng1426