Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

Hyo Chol Ha, Krishna Juluri, Yan Zhou, Steve Leung, Monika Hermankova, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.

Original languageEnglish (US)
Pages (from-to)3364-3368
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number6
DOIs
StatePublished - Mar 13 2001

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HIV-1
DNA Breaks
Fibroblasts
Infection
Adenosine Diphosphate Ribose
Virus Integration
Integrases
Virus Diseases
Nuclear Proteins
HIV Infections
Genome
Enzymes
Poly (ADP-Ribose) Polymerase-1
Therapeutics
Poly(ADP-ribose) Polymerase Inhibitors

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration. / Ha, Hyo Chol; Juluri, Krishna; Zhou, Yan; Leung, Steve; Hermankova, Monika; Snyder, Solomon H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 6, 13.03.2001, p. 3364-3368.

Research output: Contribution to journalArticle

Ha, Hyo Chol ; Juluri, Krishna ; Zhou, Yan ; Leung, Steve ; Hermankova, Monika ; Snyder, Solomon H. / Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 6. pp. 3364-3368.
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