Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

Hyo Chol Ha, Krishna Juluri, Yan Zhou, Steve Leung, Monika Hermankova, Solomon H. Snyder

Research output: Contribution to journalArticle

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.

Original languageEnglish (US)
Pages (from-to)3364-3368
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number6
DOIs
StatePublished - Mar 13 2001

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