Poly(β-amino esters): Procedures for synthesis and gene delivery

Jordan J. Green, Gregory T. Zugates, Robert Langer, Daniel G. Anderson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Summary: Non-viral gene delivery systems are promising as they avoid many problems of viral gene therapy by having increased design flexibility, high safety, large DNA cargo capacity, and ease of manufacture. Here, we describe the use of polymeric vectors, in particular biodegradable poly(β-amino esters) (PBAEs), for non-viral gene delivery. These polymers are able to self-assemble with DNA and form positively charged gene delivery nanoparticles. Methods for synthesis of these polymers, particle self-assembly, and transfection using these particles are delineated. A standard protocol is presented as well as a high-throughput screening technique that can be used to more quickly optimize transfection parameters for efficient delivery.

Original languageEnglish (US)
Title of host publicationMacromolecular Drug Delivery
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages53-63
Number of pages11
ISBN (Print)9781588299994
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume480
ISSN (Print)1064-3745

Keywords

  • Drug delivery
  • Gene therapy
  • High-throughput screening
  • Polymers
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Green, J. J., Zugates, G. T., Langer, R., & Anderson, D. G. (2009). Poly(β-amino esters): Procedures for synthesis and gene delivery. In Macromolecular Drug Delivery: Methods and Protocols (pp. 53-63). (Methods in Molecular Biology; Vol. 480). Humana Press. https://doi.org/10.1007/978-1-59745-429-2_4