Poly(β-amino ester)-DNA complexes: Time-resolved fluorescence and cellular transfection studies

Elina Vuorimaa; Tiia Maaria Ketola; Jordan J. Green; Martina Hanzlíková; Helge Lemmetyinen; Robert Langer; Daniel G. Anderson; Arto Urtti; Marjo Yliperttula

doi:10.1016/j.jconrel.2011.06.016

Poly(β-amino ester)-DNA complexes: Time-resolved fluorescence and cellular transfection studies

Elina Vuorimaa, Tiia Maaria Ketola, Jordan J. Green, Martina Hanzlíková, Helge Lemmetyinen, Robert Langer, Daniel G. Anderson, Arto Urtti, Marjo Yliperttula

School of Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.

Original language	English (US)
Pages (from-to)	171-176
Number of pages	6
Journal	Journal of Controlled Release
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/j.jconrel.2011.06.016
State	Published - Sep 5 2011

Keywords

DNA binding
DNA complexation
Gene delivery
Poly(β-amino esters)
Time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

Pharmaceutical Science

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10.1016/j.jconrel.2011.06.016

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title = "Poly(β-amino ester)-DNA complexes: Time-resolved fluorescence and cellular transfection studies",

abstract = "A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.",

keywords = "DNA binding, DNA complexation, Gene delivery, Poly(β-amino esters), Time-resolved fluorescence spectroscopy",

author = "Elina Vuorimaa and Ketola, {Tiia Maaria} and Green, {Jordan J.} and Martina Hanzl{\'i}kov{\'a} and Helge Lemmetyinen and Robert Langer and Anderson, {Daniel G.} and Arto Urtti and Marjo Yliperttula",

note = "Funding Information: This work was supported by the Academy of Finland and by NIH grants CA132091 and CA115527 .",

year = "2011",

month = sep,

day = "5",

doi = "10.1016/j.jconrel.2011.06.016",

language = "English (US)",

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AU - Vuorimaa, Elina

AU - Ketola, Tiia Maaria

AU - Green, Jordan J.

AU - Hanzlíková, Martina

AU - Lemmetyinen, Helge

AU - Langer, Robert

AU - Anderson, Daniel G.

AU - Urtti, Arto

AU - Yliperttula, Marjo

N1 - Funding Information: This work was supported by the Academy of Finland and by NIH grants CA132091 and CA115527 .

PY - 2011/9/5

Y1 - 2011/9/5

N2 - A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.

AB - A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.

KW - DNA binding

KW - DNA complexation

KW - Gene delivery

KW - Poly(β-amino esters)

KW - Time-resolved fluorescence spectroscopy

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Poly(β-amino ester)-DNA complexes: Time-resolved fluorescence and cellular transfection studies

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