Poly(β-amino ester)-DNA complexes: Time-resolved fluorescence and cellular transfection studies

Elina Vuorimaa, Tiia Maaria Ketola, Jordan J. Green, Martina Hanzlíková, Helge Lemmetyinen, Robert Langer, Daniel G. Anderson, Arto Urtti, Marjo Yliperttula

Research output: Contribution to journalArticle

Abstract

A large number of different polymers have been developed and studied for application as DNA carriers for non-viral gene delivery, but the DNA binding properties are not understood. This study describes the efficiency of nanoparticle formation by time-resolved fluorescence measurements for poly(β-amino esters), cationic biodegradable polymers with DNA complexation and transfection capability. From the large library of poly(β-amino esters) ten polymers with different transfection efficacies were chosen for this study. The binding constants for nanoparticle formation were determined and compared to with the same method. Although the DNA binding efficiency of the amine groups are similar for both types of polymers, the overall binding constants are an order of magnitude smaller for poly(β-amino esters) than for 25 kDa polyethylenimines, yet poly(β-amino esters) show comparable DNA transfection efficacy with polyethylenimines. Within this series of polymers the transfection efficacy showed increasing trend in association with relative efficiency of nanoparticle formation.

Original languageEnglish (US)
Pages (from-to)171-176
Number of pages6
JournalJournal of Controlled Release
Volume154
Issue number2
DOIs
StatePublished - Sep 5 2011

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Keywords

  • DNA binding
  • DNA complexation
  • Gene delivery
  • Poly(β-amino esters)
  • Time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Vuorimaa, E., Ketola, T. M., Green, J. J., Hanzlíková, M., Lemmetyinen, H., Langer, R., Anderson, D. G., Urtti, A., & Yliperttula, M. (2011). Poly(β-amino ester)-DNA complexes: Time-resolved fluorescence and cellular transfection studies. Journal of Controlled Release, 154(2), 171-176. https://doi.org/10.1016/j.jconrel.2011.06.016