Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis

Andrew Holland, Daniele Fachinetti, Sandrine Da Cruz, Quan Zhu, Benjamin Vitre, Mariana Lince-Faria, Denaly Chen, Nicole Parish, Inder M. Verma, Monica Bettencourt-Dias, Don W. Cleveland

Research output: Contribution to journalArticle

Abstract

Centrioles organize the centrosome, and accurate control of their number is critical for the maintenance of genomic integrity. Centrioles duplicate once per cell cycle, and duplication is coordinated by Polo-like kinase 4 (Plk4). We previously demonstrated that Plk4 accumulation is autoregulated by its own kinase activity. However, loss of heterozygosity of Plk4 in mouse embryonic fibroblasts has been proposed to cause cytokinesis failure as a primary event, leading to centrosome amplification and gross chromosomal abnormalities. Using targeted gene disruption, we show that human epithelial cells with one inactivated Plk4 allele undergo neither cytokinesis failure nor increase in centrosome amplification. Plk4 is shown to localize exclusively at the centrosome, with none in the spindle midbody. Substantial depletion of Plk4 by small interfering RNA leads to loss of centrioles and subsequent spindle defects that lead to a modest increase in the rate of cytokinesis failure. Therefore, Plk4 is a centriole-localized kinase that does not directly regulate cytokinesis.

Original languageEnglish (US)
Pages (from-to)1838-1845
Number of pages8
JournalMolecular Biology of the Cell
Volume23
Issue number10
DOIs
StatePublished - May 15 2012
Externally publishedYes

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Centrioles
Cytokinesis
Phosphotransferases
Centrosome
Loss of Heterozygosity
Chromosome Aberrations
Small Interfering RNA
Cell Cycle
Fibroblasts
Epithelial Cells
Alleles
Maintenance

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Holland, A., Fachinetti, D., Da Cruz, S., Zhu, Q., Vitre, B., Lince-Faria, M., ... Cleveland, D. W. (2012). Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis. Molecular Biology of the Cell, 23(10), 1838-1845. https://doi.org/10.1091/mbc.E11-12-1043

Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis. / Holland, Andrew; Fachinetti, Daniele; Da Cruz, Sandrine; Zhu, Quan; Vitre, Benjamin; Lince-Faria, Mariana; Chen, Denaly; Parish, Nicole; Verma, Inder M.; Bettencourt-Dias, Monica; Cleveland, Don W.

In: Molecular Biology of the Cell, Vol. 23, No. 10, 15.05.2012, p. 1838-1845.

Research output: Contribution to journalArticle

Holland, A, Fachinetti, D, Da Cruz, S, Zhu, Q, Vitre, B, Lince-Faria, M, Chen, D, Parish, N, Verma, IM, Bettencourt-Dias, M & Cleveland, DW 2012, 'Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis', Molecular Biology of the Cell, vol. 23, no. 10, pp. 1838-1845. https://doi.org/10.1091/mbc.E11-12-1043
Holland, Andrew ; Fachinetti, Daniele ; Da Cruz, Sandrine ; Zhu, Quan ; Vitre, Benjamin ; Lince-Faria, Mariana ; Chen, Denaly ; Parish, Nicole ; Verma, Inder M. ; Bettencourt-Dias, Monica ; Cleveland, Don W. / Polo-like kinase 4 controls centriole duplication but does not directly regulate cytokinesis. In: Molecular Biology of the Cell. 2012 ; Vol. 23, No. 10. pp. 1838-1845.
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