Polarized fluorescence for skin cancer diagnostic with a multi-aperture camera

Haripriya Kandimalla, Jessica C. Ramella-Roman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Polarized fluorescence has shown some promising results in assessment of skin cancer margins. Researchers have used tetracycline and cross polarization imaging for nonmelanoma skin cancer demarcation as well as investigating endogenous skin polarized fluorescence. In this paper we present a new instrument for polarized fluorescence imaging, able to calculate the full fluorescence Stokes vector in one snapshot. The core of our system is a multi-aperture camera constructed with a two by two lenslet array. Three of the lenses have polarizing elements in front of them, oriented at 0°, + 45°and 90°with respect to light source polarization. A flash lamp combined with a polarizer parallel to the source-camera-sample plane and a UV filter is used as an excitation source. A blue filter in front of the camera system is used to collect only the fluorescent emission of interest and filter out the incident light. In-vitro tests of endogenous and exogenous polarized fluorescence on collagen rich material like bovine tendon were performed and Stokes vector of polarized fluorescence calculated. The system has the advantage of eliminating moving artifacts with the collection of different polarization states and stoke vector in a single snap shot.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6842
DOIs
Publication statusPublished - 2008
Externally publishedYes
EventPhotonic Therapeutics and Diagnostics IV - San Jose, CA, United States
Duration: Jan 19 2008Jan 19 2008

Other

OtherPhotonic Therapeutics and Diagnostics IV
CountryUnited States
CitySan Jose, CA
Period1/19/081/19/08

    Fingerprint

Keywords

  • Polarimetry

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kandimalla, H., & Ramella-Roman, J. C. (2008). Polarized fluorescence for skin cancer diagnostic with a multi-aperture camera. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6842). [68420J] https://doi.org/10.1117/12.768536