Polarized expression of cytokines in cell conjugates of helper T cells and splenic B cells

Abraham Kupfer, Tim R. Mosmann, Hanna Kupfer

Research output: Contribution to journalArticle

Abstract

We describe the intracellular localization, by double immunofluorescence microscopy, of four cytokines that were produced during the prolonged interaction of cloned helper T cells with resting splenic B cells. When two rabbit immunooglobulin-specific helper-T-cell clones were mixed, either separately or together, with splenic B cells in the presence of the antigen rabbit anti-mouse immunoglobulin antibodies, stable T-cell-B-cell conjugates were seen up to 29 hr later. Microscopic observations of these cells revealed that Interferon γ and interleukin 2, inside one of the T-cell clones, and interleukins 4 and 5, inside the other T-cell clone, were concentrated very close to the T-cell-B-cell contact area. The cytokines were not seen in the T cells prior to their interaction with the B cells and their production was strictly antigen-specific. These studies show, at the single-cell level, that helper-T-cell clones can remain bound to splenic B cells long enough for the T cells to produce cytokines, which are synthesized near the bound B cells. We propose that the polarized synthesis of the cytokines may result in their directed secretion toward the bound B cells. By locally secreting the cytokines, which are not antigen-specific, at the contacting T-cell-B-cell membranes, where T- and B-cell surface receptors are engaged and clustered, the helper T cells can induce selective and specific B-cell responses.

Original languageEnglish (US)
Pages (from-to)775-779
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number3
StatePublished - 1991
Externally publishedYes

Fingerprint

Helper-Inducer T-Lymphocytes
B-Lymphocytes
Cytokines
T-Lymphocytes
Clone Cells
Antigens
Rabbits
Interleukin-5
Cell Surface Receptors
Fluorescence Microscopy
Interleukin-4
Interferons
Interleukin-2
Immunoglobulins
Cell Membrane
Antibodies

Keywords

  • Cell differcntiation
  • Cell-cell interaction
  • Directed secretion
  • Double immunofluorescence microscopy

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Polarized expression of cytokines in cell conjugates of helper T cells and splenic B cells. / Kupfer, Abraham; Mosmann, Tim R.; Kupfer, Hanna.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 3, 1991, p. 775-779.

Research output: Contribution to journalArticle

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