Polarized distribution of membrane components on two-cell mouse embryos

Alan H. Handyside, Michael A Edidin, David E. Wolf

Research output: Contribution to journalArticle

Abstract

In two-cell mouse embryos, membrane components detected by a variety of antisera, lectins and lipid analogues and covalent labeling were found to be localized in the poles of the two blastomeres opposite the cleavage furrow. The proportion of polarized blastomeres increased rapidly during the first 4-5 h following first cleavage and then diminished approximately two-fold over the remaining period before second cleavage. Concurrent with this decrease in percent polarization, observed poles were found to be less spatially restricted. This polarization is not the result of a ligand induced capping or a manifestation of differences in surface topography. In light of recent measurements of lateral diffusion, the polarization of membrane components may be significant for the formation of morphogenetic gradients during cleavage.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalRoux's Archives of Developmental Biology
Volume196
Issue number5
DOIs
StatePublished - Jul 1987

Fingerprint

Blastomeres
blastomeres
cleavage
embryo
embryo (animal)
Embryonic Structures
membrane
Membranes
polarization
mice
furrows
Lectins
lectins
topography
antiserum
Immune Sera
cells
Ligands
Lipids
capping

Keywords

  • Cell polarization
  • Lectin binding
  • Lipid analogues
  • Mouse embryos
  • Stage-specific antigens

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Polarized distribution of membrane components on two-cell mouse embryos. / Handyside, Alan H.; Edidin, Michael A; Wolf, David E.

In: Roux's Archives of Developmental Biology, Vol. 196, No. 5, 07.1987, p. 273-278.

Research output: Contribution to journalArticle

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