Polarized activities of AMPK and BRSK in primary hippocampal neurons

Vedangi Sample, Santosh Ramamurthy, Kirill Gorshkov, Gabriele V. Ronnett, Jin Zhang

Research output: Contribution to journalArticle

Abstract

5'-Adenosine monophosphate-activated protein kinase (AMPK) is a master metabolic regulator that has been shown to inhibit the establishment of neuronal polarity/axogenesis under energy stress conditions, whereas brain-specific kinase (BRSK) promotes the establishment of axon-dendrite polarity and synaptic development. However, little information exists regarding the localized activity and regulation of these kinases in developing neurons. In this study, using a fluorescence resonance energy transfer (FRET)-based activity reporter that responds to both AMPK and BRSK, we found that BRSK activity is elevated in the distal region of axons in polarized hippocampal neurons before any stimulation and does not respond to either Ca2+ or 2-deoxyglucose (2-DG) stimulation. In contrast, AMPK activity is stimulated by either Ca2+ or 2-DG in the soma, dendrites, and axons of hippocampal neurons, with maximal stimulated activity observed in the distal region of the axon. Our study shows that the activities of both AMPK and BRSK are polarized in developing hippocampal neurons, with high levels in the distal region of extended axons.

Original languageEnglish (US)
Pages (from-to)1935-1946
Number of pages12
JournalMolecular Biology of the Cell
Volume26
Issue number10
DOIs
StatePublished - May 15 2015

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EphA5 Receptor
Adenosine Monophosphate
Protein Kinases
Axons
Neurons
Deoxyglucose
Dendrites
Body Patterning
Fluorescence Resonance Energy Transfer
Carisoprodol
Phosphotransferases

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Sample, V., Ramamurthy, S., Gorshkov, K., Ronnett, G. V., & Zhang, J. (2015). Polarized activities of AMPK and BRSK in primary hippocampal neurons. Molecular Biology of the Cell, 26(10), 1935-1946. https://doi.org/10.1091/mbc.E14-02-0764)

Polarized activities of AMPK and BRSK in primary hippocampal neurons. / Sample, Vedangi; Ramamurthy, Santosh; Gorshkov, Kirill; Ronnett, Gabriele V.; Zhang, Jin.

In: Molecular Biology of the Cell, Vol. 26, No. 10, 15.05.2015, p. 1935-1946.

Research output: Contribution to journalArticle

Sample, V, Ramamurthy, S, Gorshkov, K, Ronnett, GV & Zhang, J 2015, 'Polarized activities of AMPK and BRSK in primary hippocampal neurons', Molecular Biology of the Cell, vol. 26, no. 10, pp. 1935-1946. https://doi.org/10.1091/mbc.E14-02-0764)
Sample, Vedangi ; Ramamurthy, Santosh ; Gorshkov, Kirill ; Ronnett, Gabriele V. ; Zhang, Jin. / Polarized activities of AMPK and BRSK in primary hippocampal neurons. In: Molecular Biology of the Cell. 2015 ; Vol. 26, No. 10. pp. 1935-1946.
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