Polarization of the anterior-posterior axis of C. Elegans is a microtubule-directed process

Matthew R. Wallenfang, Geraldine Seydoux

Research output: Contribution to journalArticle

Abstract

In Caenorhabditis elegans, polarity along the anterior-posterior (A/P) axis is established shortly after fertilization and is determined by the sperm, whose position specifies the posterior end of the embryo. Although many factors required for the establishment of A/P polarity have been described, the nature of the spatial cue provided by the sperm remains unknown. Here we show that a microtubule-organizing centre is necessary and sufficient to establish several aspects of A/P polarity. In wild-type embryos, appearance of the first molecular asymmetries along the A/P axis correlates with and requires nucleation of microtubules by the sperm-derived centrosomes (sperm asters). In mutant embryos arrested in meiosis, sperm asters fail to form, and posterior is defined by the position of the persistent meiotic spindle rather than by the position of the sperm. Together, our data indicate that the primary spatial cue for A/P polarity in C. Elegans derives from microtubules emanating from the sperm asters. Our findings support a parallel between C. Elegans zygotes and other cells, such as Drosophila oocytes, which rely on microtubules to regulate polarity.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalNature
Volume408
Issue number6808
DOIs
StatePublished - Nov 2 2000

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Polarization of the anterior-posterior axis of C. Elegans is a microtubule-directed process'. Together they form a unique fingerprint.

  • Cite this