Abstract
The GTP-binding regulatory proteins (G proteins) in the membranes of the lung parenchyma from normal, uninfected ferrets were compared to those from immunosuppressed animals with and without Pneumocystis carinii pneumonitis. In lung membranes, pertussis toxin (PT) catalyzed ADP ribosylation of a 41- kDa protein; treatment with cholera toxin (CT) led to ribosylation of a 44- kDa polypeptide. Compared to that in the normal ferrets, the level of the 44- kDa protein was dramatically suppressed in the P. carinii-infected animals. Western blot analysis with specific antibodies to α(s) (which recognized CT substrates), α(common) (which reacted to PT substrates), the α(q/11) epitope, and the β subunit demonstrated that these proteins were decreased in animals with P. carinii pneumonitis (PCP). Western blotting of PCP-free membranes with a pan-Ras antibody revealed a 21-kDa polypeptide (corresponding to small G proteins). The level of the 21-kDa protein in membranes from PCP-affected animals was only 30% of that in membranes from PCP-free animals. Finally, analogous studies performed with rat lung membranes revealed similar findings. These data suggest that, independent of its exacerbation of immunosuppression. PCP leads to extensive changes in the GTP-binding proteins in the lung.
Original language | English (US) |
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Pages (from-to) | 679-685 |
Number of pages | 7 |
Journal | Journal of Parasitology |
Volume | 83 |
Issue number | 4 |
DOIs | |
State | Published - Aug 1997 |
Externally published | Yes |
ASJC Scopus subject areas
- Parasitology
- Ecology, Evolution, Behavior and Systematics