Pneumocvstis carinii infection alters GTP-binding proteins in lungs

Helieh S. Oz, Walter T. Hughes

Research output: Contribution to journalArticle

Abstract

Pneumocvstis carinii is a common cause of life-threatening pneumonitis in the acquired immunodeficiency syndrome (AIDS),and other immuncompromised patients. The GTP-binding proteins were compared in parenchyma! lung membrane preparations from normal; immunosuppressed without P. carinii pneumonitis (PCP); and, immunosuppressed with PCP ferrets and rats. Pertussis toxin (PT) and cholera toxin (CT) ADP-ribosylated respectively a 41kDa, and two distinct 44/48KDa polypeptides in lung fractions. The 44/48KDa proteins were highly suppressed in PCP-infected compared to PCP-free membranes. Western blot analysis using specific antibodies to G-proteins, a, to detect CT, acomm ,.2 0 for PT substrates, Gq/a|, and subunit, exposed these proteins. PCP altered these proteins. Pan-ras antibody revealed a 21 KJDa peptide corresponding to small G-proteins in PCP-free, however PCP membranes expressed only 30% of this peptide. Comparison of studies performed with ferret and rat lung membranes revealed similar findings with only minor differences. These data are evidence for extensive changes induced by PCP in the GTP-binding proteins independent of the state of immunosuppression.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

G-proteins
GTP-Binding Proteins
pneumonia
Pneumonia
lungs
Membranes
Lung
Cholera Toxin
Pertussis Toxin
Infection
infection
Peptides
Rats
Proteins
Monomeric GTP-Binding Proteins
Antibodies
pertussis toxin
Ferrets
cholera toxin
ferrets

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Pneumocvstis carinii infection alters GTP-binding proteins in lungs. / Oz, Helieh S.; Hughes, Walter T.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Oz, Helieh S. ; Hughes, Walter T. / Pneumocvstis carinii infection alters GTP-binding proteins in lungs. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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