TY - JOUR
T1 - Pneumatically Sprayed Gold Nanoparticles for Mass Spectrometry Imaging of Neurotransmitters
AU - McLaughlin, Nolan
AU - Bielinski, Tyler M.
AU - Tressler, Caitlin M.
AU - Barton, Eric
AU - Glunde, Kristine
AU - Stumpo, Katherine A.
N1 - Funding Information:
N.M., T.M.B., and K.A.S. would like to thank the University of Scranton and the Chemistry Department for providing facilities, equipment, and financial support to conduct these experiments. N.M. and K.A.S. would like to thank the University of New Hampshire and Drs. David Ashline and Vernon Reinhold for the use of their Kratos Axima MALDI-TOF MS. K.A.S. would like to thank Dr. Timothy Foley, Alysse Machalek, and Stefan Olsen at the University of Scranton for assistance with the neuroblastoma cell line. The authors would like to thank the Johns Hopkins Applied Imaging Mass Spectrometry (AIMS) Core Facility at the Johns Hopkins University School of Medicine for undertaking the MALDI and LDI imaging in this project.
Publisher Copyright:
© American Society for Mass Spectrometry. Published by the American Chemical Society. All rights reserved.
PY - 2020/12/2
Y1 - 2020/12/2
N2 - Using citrate-capped gold nanoparticles (AuNPs) for laser desorption ionization mass spectrometry (LDI-MS) is an approach that has demonstrated broad applicability to ionization of different classes of molecules. Here, we show a simple AuNP-based approach for the ionization of neurotransmitters. Specifically, the detection of acetylcholine, dopamine, epinephrine, glutamine, 4-aminobutyric acid, norepinephrine, octopamine, and serotonin was achieved at physiologically relevant concentrations in serum and homogenized tissue. Additionally, pneumatic spraying of AuNPs onto tissue sections facilitated mass spectrometry imaging (MSI) of rabbit brain tissue sections, zebrafish embryos, and neuroblastoma cells for several neurotransmitters simultaneously using this quick and simple sample preparation. AuNP LDI-MS achieved mapping of neurotransmitters in fine structures of zebrafish embryos and neuroblastoma cells at a lateral spatial resolution of 5 μm. The use of AuNPs to ionize small aminergic neurotransmitters in situ provides a fast, high-spatial resolution method for simultaneous detection of a class of molecules that typically evade comprehensive detection with traditional matrixes.
AB - Using citrate-capped gold nanoparticles (AuNPs) for laser desorption ionization mass spectrometry (LDI-MS) is an approach that has demonstrated broad applicability to ionization of different classes of molecules. Here, we show a simple AuNP-based approach for the ionization of neurotransmitters. Specifically, the detection of acetylcholine, dopamine, epinephrine, glutamine, 4-aminobutyric acid, norepinephrine, octopamine, and serotonin was achieved at physiologically relevant concentrations in serum and homogenized tissue. Additionally, pneumatic spraying of AuNPs onto tissue sections facilitated mass spectrometry imaging (MSI) of rabbit brain tissue sections, zebrafish embryos, and neuroblastoma cells for several neurotransmitters simultaneously using this quick and simple sample preparation. AuNP LDI-MS achieved mapping of neurotransmitters in fine structures of zebrafish embryos and neuroblastoma cells at a lateral spatial resolution of 5 μm. The use of AuNPs to ionize small aminergic neurotransmitters in situ provides a fast, high-spatial resolution method for simultaneous detection of a class of molecules that typically evade comprehensive detection with traditional matrixes.
KW - gold nanoparticles
KW - mass spectrometry imaging
KW - neuroblastoma
KW - neurotransmitters
KW - zebrafish
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U2 - 10.1021/jasms.0c00156
DO - 10.1021/jasms.0c00156
M3 - Article
C2 - 32841002
AN - SCOPUS:85098332020
SN - 1044-0305
VL - 31
SP - 2452
EP - 2461
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 12
ER -