Plus strand strong-stop DNA transfer in yeast Ty retrotransposons

Vit Lauermann, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

The yeast Ty1 LTR retrotransposon replicates by reverse transcription and integration; the process shows many similarities to the retroviral life cycle. However, we show that plus strand strong-stop DNA transfer in yeast Ty1 elements differs from the analogous retroviral process. By analysis of the native structure of the Ty1 primer binding site and by a series of manipulations of this region and assessment of the effects on retrotransposition, we show that primer binding site inheritance is not from the tRNA primer, which is inconsistent with classical retroviral models. This unusual inheritance pattern holds even when the Ty1 primer binding site is lengthened in order to be more retrovirus-like. Finally, the distantly related Ty3 element has an inheritance pattern like Ty1, indicating evolutionary conservation of the alternative pathway used by Ty1. Based on these results we arrive at a plus strand primer recycling model that explains Ty1 plus strand strong-stop DNA transfer and inheritance patterns in the primer binding site.

Original languageEnglish (US)
Pages (from-to)6603-6612
Number of pages10
JournalThe EMBO journal
Volume16
Issue number21
DOIs
StatePublished - 1997

Fingerprint

Retroelements
Inheritance Patterns
Yeast
Yeasts
Binding Sites
Recycling
Retroviridae
Transcription
Transfer RNA
Life Cycle Stages
Reverse Transcription
Life cycle
Conservation
strong-stop DNA

Keywords

  • Evolution
  • Primer-binding site
  • Retrotransposon
  • Reverse transcriptase
  • tRNA
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Plus strand strong-stop DNA transfer in yeast Ty retrotransposons. / Lauermann, Vit; Boeke, Jef D.

In: The EMBO journal, Vol. 16, No. 21, 1997, p. 6603-6612.

Research output: Contribution to journalArticle

Lauermann, Vit ; Boeke, Jef D. / Plus strand strong-stop DNA transfer in yeast Ty retrotransposons. In: The EMBO journal. 1997 ; Vol. 16, No. 21. pp. 6603-6612.
@article{10f8ada2877646488aa3158077d46c6e,
title = "Plus strand strong-stop DNA transfer in yeast Ty retrotransposons",
abstract = "The yeast Ty1 LTR retrotransposon replicates by reverse transcription and integration; the process shows many similarities to the retroviral life cycle. However, we show that plus strand strong-stop DNA transfer in yeast Ty1 elements differs from the analogous retroviral process. By analysis of the native structure of the Ty1 primer binding site and by a series of manipulations of this region and assessment of the effects on retrotransposition, we show that primer binding site inheritance is not from the tRNA primer, which is inconsistent with classical retroviral models. This unusual inheritance pattern holds even when the Ty1 primer binding site is lengthened in order to be more retrovirus-like. Finally, the distantly related Ty3 element has an inheritance pattern like Ty1, indicating evolutionary conservation of the alternative pathway used by Ty1. Based on these results we arrive at a plus strand primer recycling model that explains Ty1 plus strand strong-stop DNA transfer and inheritance patterns in the primer binding site.",
keywords = "Evolution, Primer-binding site, Retrotransposon, Reverse transcriptase, tRNA, Yeast",
author = "Vit Lauermann and Boeke, {Jef D.}",
year = "1997",
doi = "10.1093/emboj/16.21.6603",
language = "English (US)",
volume = "16",
pages = "6603--6612",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "21",

}

TY - JOUR

T1 - Plus strand strong-stop DNA transfer in yeast Ty retrotransposons

AU - Lauermann, Vit

AU - Boeke, Jef D.

PY - 1997

Y1 - 1997

N2 - The yeast Ty1 LTR retrotransposon replicates by reverse transcription and integration; the process shows many similarities to the retroviral life cycle. However, we show that plus strand strong-stop DNA transfer in yeast Ty1 elements differs from the analogous retroviral process. By analysis of the native structure of the Ty1 primer binding site and by a series of manipulations of this region and assessment of the effects on retrotransposition, we show that primer binding site inheritance is not from the tRNA primer, which is inconsistent with classical retroviral models. This unusual inheritance pattern holds even when the Ty1 primer binding site is lengthened in order to be more retrovirus-like. Finally, the distantly related Ty3 element has an inheritance pattern like Ty1, indicating evolutionary conservation of the alternative pathway used by Ty1. Based on these results we arrive at a plus strand primer recycling model that explains Ty1 plus strand strong-stop DNA transfer and inheritance patterns in the primer binding site.

AB - The yeast Ty1 LTR retrotransposon replicates by reverse transcription and integration; the process shows many similarities to the retroviral life cycle. However, we show that plus strand strong-stop DNA transfer in yeast Ty1 elements differs from the analogous retroviral process. By analysis of the native structure of the Ty1 primer binding site and by a series of manipulations of this region and assessment of the effects on retrotransposition, we show that primer binding site inheritance is not from the tRNA primer, which is inconsistent with classical retroviral models. This unusual inheritance pattern holds even when the Ty1 primer binding site is lengthened in order to be more retrovirus-like. Finally, the distantly related Ty3 element has an inheritance pattern like Ty1, indicating evolutionary conservation of the alternative pathway used by Ty1. Based on these results we arrive at a plus strand primer recycling model that explains Ty1 plus strand strong-stop DNA transfer and inheritance patterns in the primer binding site.

KW - Evolution

KW - Primer-binding site

KW - Retrotransposon

KW - Reverse transcriptase

KW - tRNA

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=0030776187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030776187&partnerID=8YFLogxK

U2 - 10.1093/emboj/16.21.6603

DO - 10.1093/emboj/16.21.6603

M3 - Article

VL - 16

SP - 6603

EP - 6612

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 21

ER -