Pluripotent fates and tissue regenerative potential of adult olfactory bulb neural stem and progenitor cells

Zhiping Liu, Lee J Martin

Research output: Contribution to journalArticle

Abstract

Neural stem cells and progenitor cells reside in the adult olfactory bulb (OB) core of mouse, rat, and human. Adult rodent OB core cells have the capacities for self-renewal and multipotency and form neurospheres. The differentiation fates of these neurosphere-forming cells were studied in vitro and in vivo. Adult OB neurospheres were comprised of stem cells and neuronal and glial progenitor cells. OB neurospheres in co-culture with primary embryonic striatal neurons and cortical neurons generated cells with morphological and neurochemical phenotypes of striatal and cortical neurons, respectively. Transplanted OB cells, delivered as dissociated cells or as intact neurospheres, dispersed, survived for long-term, extended neuntes, migrated, expressed neuronal or glial markers, and formed synapses with host neurons when placed into the environment of the nonlesioned and lesioned central nervous system (CNS). Grafted cells in the CNS also showed angiogenic capacity by forming blood vessels. In a model of spinal motor neuron degeneration, adult OB neurosphere cells transplanted into lesioned spinal cord adopted phenotypes of motor neurons and had a robust potential to become oligodendrocytes. OB core cells in co-culture with skeletal myoblasts generated skeletal muscle cells. Chimeric skeletal muscle was formed when mouse OB neurospheres were transplanted into rat skeletal muscle. Within skeletal muscle, adult OB neurosphere cells became myogenic progenitor cells to form myotubes de novo. We conclude that the adult mammalian OB is a source of pluripotent neural stem cells and progenitor cells that have the potential to become, in a context-dependent manner, specific types of cells for regeneration of tissues in brain, spinal cord, and muscle.

Original languageEnglish (US)
Pages (from-to)1479-1499
Number of pages21
JournalJournal of Neurotrauma
Volume21
Issue number10
DOIs
StatePublished - Oct 2004

Fingerprint

Neural Stem Cells
Olfactory Bulb
Stem Cells
Skeletal Muscle
Neurons
Corpus Striatum
Motor Neurons
Coculture Techniques
Neuroglia
Spinal Cord
Central Nervous System
Skeletal Myoblasts
Phenotype
Nerve Degeneration
Pluripotent Stem Cells
Skeletal Muscle Fibers
Oligodendroglia
Synapses
Muscle Cells
Blood Vessels

Keywords

  • Alzheimer's disease
  • Amyotrophic lateral sclerosis
  • Brain trauma
  • Motor neuron
  • Muscular dystrophy
  • Myogenesis
  • Neuronal replacement
  • Spinal cord injury
  • Stem cell therapy
  • White matter

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Pluripotent fates and tissue regenerative potential of adult olfactory bulb neural stem and progenitor cells. / Liu, Zhiping; Martin, Lee J.

In: Journal of Neurotrauma, Vol. 21, No. 10, 10.2004, p. 1479-1499.

Research output: Contribution to journalArticle

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