Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy

Young K. Sung, Cheil Moon, Joo Yeon Yoo, Chanil Moon, David B Pearse, Jonathan A. Pevsner, Gabriele V. Ronnett

Research output: Contribution to journalArticle

Abstract

Subtraction suppression hybridization was used with high throughput screening to identify transcripts of genes that are differentially expressed in nasal epithelium following lesioning of the olfactory bulb, termed bulbectomy. We isolated the rat homologue of plunc, a murine gene highly expressed in lung and nasopharyngeal regions, by this method. Rat plunc encodes a 270-amino acid protein containing a putative signal peptide. plunc up-regulation in respiratory epithelium was confirmed by Northern blot and in situ hybridization. plunc mRNA was expressed in nasal epithelium, heart, lung, thymus, and salivary gland in adult rodent. plunc was expressed in nasal epithelium, thymus, and salivary gland during embryogenesis. Antibodies against Plunc detected a 31-kDa protein in lung, heart, and spleen. Rat nasal epithelium displayed robust immunoreactivity that was highly localized to the microvilli layer of respiratory epithelium. The expression of plunc was up-regulated after bulbectomy in respiratory epithelium. We also detected secreted plunc in rat and human mucus. Sequence and homology analyses suggest that Plunc is a member of the secretory gland protein family with putative bactericidal/bacteriostatic function. This is the first protein found in respiratory epithelium whose expression is regulated by olfactory neuronal injury and may provide protection against infection subsequent to injury.

Original languageEnglish (US)
Pages (from-to)12762-12769
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number15
DOIs
StatePublished - Apr 12 2002

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Respiratory Mucosa
Nasal Mucosa
Rats
Thymus
Salivary Glands
Lung
Thymus Gland
Proteins
Genes
Olfactory Bulb
Wounds and Injuries
Mucus
Sequence Homology
Microvilli
Protein Sorting Signals
Northern Blotting
Embryonic Development
In Situ Hybridization
Sequence Analysis
Rodentia

ASJC Scopus subject areas

  • Biochemistry

Cite this

Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy. / Sung, Young K.; Moon, Cheil; Yoo, Joo Yeon; Moon, Chanil; Pearse, David B; Pevsner, Jonathan A.; Ronnett, Gabriele V.

In: Journal of Biological Chemistry, Vol. 277, No. 15, 12.04.2002, p. 12762-12769.

Research output: Contribution to journalArticle

Sung, Young K. ; Moon, Cheil ; Yoo, Joo Yeon ; Moon, Chanil ; Pearse, David B ; Pevsner, Jonathan A. ; Ronnett, Gabriele V. / Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 15. pp. 12762-12769.
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AU - Pearse, David B

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AU - Ronnett, Gabriele V.

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