TY - JOUR
T1 - Platelet phosphorylase activity in the presence of activators and inhibitors of aggregation
AU - Deisseroth, Albert
AU - Wolfe, Sidney M.
AU - Shulman, N. Raphael
PY - 1970/5/11
Y1 - 1970/5/11
N2 - Phosphorylase activity in suspensions of human platelets has been assayed before and after addition of thrombin and epinephrine which aggregate platelets, prostaglandins E1 and E2 and dibutyryl-3′,5′-AMP which inhibit aggregation, or sodium fluoride and isoproterenol which alter adenine nucleotide metabolism. These various reagents did not change phosphorylase a activity in intact platelets, nor did cyclic-3′,5′-AMP affect platelet phosphorylase activity in subcellular fractions. In contrast to many other tissues, platelet phosphorylase activity does not appear to be modulated by a direct effect of cyclic AMP.
AB - Phosphorylase activity in suspensions of human platelets has been assayed before and after addition of thrombin and epinephrine which aggregate platelets, prostaglandins E1 and E2 and dibutyryl-3′,5′-AMP which inhibit aggregation, or sodium fluoride and isoproterenol which alter adenine nucleotide metabolism. These various reagents did not change phosphorylase a activity in intact platelets, nor did cyclic-3′,5′-AMP affect platelet phosphorylase activity in subcellular fractions. In contrast to many other tissues, platelet phosphorylase activity does not appear to be modulated by a direct effect of cyclic AMP.
UR - http://www.scopus.com/inward/record.url?scp=0014941259&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014941259&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(70)90613-3
DO - 10.1016/0006-291X(70)90613-3
M3 - Article
C2 - 5421954
AN - SCOPUS:0014941259
SN - 0006-291X
VL - 39
SP - 551
EP - 557
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -