Platelet-endothelial cell adhesion molecule-1-directed immunotargeting to cardiopulmonary vasculature

Arnaud Scherpereel, Jonathan J. Rome, Rainer Wiewrodt, Simon C. Watkins, David Winslow Harshaw, Sean Alder, Melpo Christofidou-Solomidou, Elliott Haut, Juan Carlos Murciano, Marian Nakada, Steven M. Albelda, Vladimir R. Muzykantov

Research output: Contribution to journalArticle

Abstract

Therapeutic molecules conjugated with antibodies to the platelet-endothelial cell adhesion molecule-1 (PECAM-1) accumulate in the pulmonary endothelium after i.v. injection in mice. In this study, we characterized PECAM-directed targeting to the lung and heart after local versus systemic intravascular administration in a large animal model, pigs. Radiolabel tracing showed that 1 h post-i.v. injection, 35% of anti-PECAM versus 2.5% of control IgG had accumulated in the lungs. Infusion of anti-PECAM via a catheter placed in the right pulmonary artery (RPA) resulted in a 3-fold elevation of the uptake in the right lower lobe and 2-fold reduction of uptake in the left lobes in the lung. Cardiac uptake of anti-PECAM was negligible after i.v. and RPA infusion. In contrast, delivery with a catheter placed in the right coronary artery (RCA) resulted in a 4-fold elevation of cardiac uptake of anti-PECAM, but not IgG, compared with i.v. injection. To estimate the targeting of an active reporter enzyme, streptavidin-conjugated β3-galactosidase (β-Gal) was coupled to anti-PECAM or IgG (anti-PECAM/β-Gal and IgG/β-Gal) and injected into the RCA. β-Gal activity was markedly elevated in the heart and lungs (5- and 25-fold increased, respectively) after injection of anti-PECAM/β-Gal, but not IgG/β-Gal. Image analysis confirmed endothelial targeting of anti-PECAM/β-Gal in the heart and lung. In summary, anti-PECAM antibody conjugates deliver agents to the pulmonary endothelium regardless of injection route, whereas local arterial infusion permits targeting to the cardiac vasculature. This paradigm may be useful for drug targeting to endothelium in lungs, heart, and possibly other organs.

Original languageEnglish (US)
Pages (from-to)777-786
Number of pages10
JournalJournal of Pharmacology and Experimental Therapeutics
Volume300
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Pharmacology

Cite this

Scherpereel, A., Rome, J. J., Wiewrodt, R., Watkins, S. C., Harshaw, D. W., Alder, S., ... Muzykantov, V. R. (2002). Platelet-endothelial cell adhesion molecule-1-directed immunotargeting to cardiopulmonary vasculature. Journal of Pharmacology and Experimental Therapeutics, 300(3), 777-786. https://doi.org/10.1124/jpet.300.3.777