Platelet-derived growth factor and basic fibroblast growth factor regulate cell proliferation and the expression of notch-1 receptor in a new oligodendrocyte cell line

Carlo Colantuoni, Amy E. Purcell, Christopher M.L. Bouton, Jonathan Pevsner

Research output: Contribution to journalArticle

Abstract

We generated a new cell line, N38, by conditionally immortalizing mouse oligodendrocytes (OLs) at early stages of maturation. The morphology and marker expression pattern suggest N38 cells are similar to immature OLs. N38 cells were sensitive to changes in serum concentrations, and forcing the cells to differentiate in low serum at 39°C significantly decreased the survival of the cells. Importantly, addition of PDGFaa, bFGF or astrocyte-conditioned medium had protective effects on the cells, by increasing cell proliferation but not cell differentiation. This effect was receptor-mediated. Exposure of N38 cells to differentiating signals such as retinoic acid did not cause further differentiation of the cells. The N38 cell line expresses the vertebrate homolog of the Drosophila notch-1 receptor, a molecule that appears to regulate OL differentiation. Notch-1 receptor was homogeneously distributed in the somas of N38 cells. Incubation of N38 cells with either PDGFaa or bFGF, however, induced a polarized distribution of the receptor in the majority of the cells as well as an upregulation of receptor protein levels. The upregulation of molecules, such the notch-1 receptor, in pathways that control differentiation might be an important mechanism for keeping OL precursors in an undifferentiated state during their exit of the germinal layer and migration in the developing central nervous system. This OL cell line might constitute a suitable model for studies of regulatory mechanisms at this stage of OL differentiation. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)319-328
Number of pages10
JournalJournal of neuroscience research
Volume62
Issue number3
DOIs
StatePublished - Nov 1 2000

Keywords

  • FGF
  • Jagged-1
  • Myelin basic proteins
  • Notch-1
  • Oligodendrocytes
  • PDGF
  • Proteolipids

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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