Platelet-derived growth factor-A-induced retinal gliosis protects against ischemic retinopathy

Haruhiko Yamada, Eri Yamada, Akira Ando, Man Seong Seo, Noriko Esumi, Naoyuki Okamoto, Melissa Vinores, William LaRochelle, Donald J. Zack, Peter A. Campochiaro

Research output: Contribution to journalArticle

Abstract

Retinal astrocytes are located in the nerve fiber layer and along retinal blood vessels and have been hypothesized to participate in the induction and maintenance of the blood-retinal barrier. Platelet-derived growth factor-A (PDGF-A) is normally produced by retinal ganglion cells and is involved in astrocyte recruitment and proliferation. We used gain-of-function transgenic mice that express PDGF-A in photoreceptors to explore the roles of PDGF-A and astrocytes in the retina. Transgene-positive mice developed glial infiltration of the inner retina and had significantly less oxygen-induced retinal vascular closure and no neovascularization compared with littermate controls, which had prominent vascular closure and neovascularization. The increased survival of endothelial cells in transgenic mice in the face of oxygen-induced down-regulation of vascular endothelial growth factor was accompanied by an increase in astrocyte-derived fibroblast growth factor-2. Therefore, PDGF-A increases retinal astrocytes, which promote the survival of endothelial cells as well as their expression of barrier characteristics.

Original languageEnglish (US)
Article number64752
Pages (from-to)477-487
Number of pages11
JournalAmerican Journal of Pathology
Volume156
Issue number2
DOIs
StatePublished - Feb 2000

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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