Plate-clearing technique to screen mixed microbial populations for protein degraders

Jason A. Caplan, Jed W. Fahey

Research output: Contribution to journalArticlepeer-review

Abstract

A plate-clearing assay was developed to screen for protein-degrading microorganisms from soil and activated sludge. An insoluble protein, with a substrate-bound dye (Azocoll®) was incorporated into a nutrient agar medium. Microbial enzymatic attack on the substrate produced zones of clearing around colonies with proteolytic capability.

Original languageEnglish (US)
Pages (from-to)373-375
Number of pages3
JournalSoil Biology and Biochemistry
Volume14
Issue number4
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

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