Plasmodium yoelii: Peptide immunization induces protective CD4+ T cells against a previously unrecognized cryptic epitope of the circumsporozoite protein

Yoshiko Takita-Sonoda, Moriya Tsuji, Kamal Kamboj, Ruth S. Nussenzweig, Pedro Clavijo, Fidel Zavala

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we characterized the CD4+ T cell response directed against two distinct epitopes located in the circumsporozoite (CS) protein of Plasmodium yoelii. The immunization of mice with P. yoelii sporozoites induced CD4+ T cells which were mostly directed against one of these peptides, Py-1, previously reported to contain a CD4+ epitope. The CD4+ T cells directed against this immunodominant epitope were mostly of the Th-1 type. Another newly identified peptide, AS44, induced a specific CD4+ T cell response, which was mainly detectable after immunization with the corresponding peptide. Several CD4+ T cell clones, recognizing this epitope, were generated and their lymphokine expression was characterized, as well as their surface markers and their anti-parasite activity in vivo. It was noteworthy that some of these CD4+ T cell clones, which recognize this cryptic epitope and were of different Th subtypes, were shown to have a strong inhibitory effect on the development of liver stages of malaria parasites.

Original languageEnglish (US)
Pages (from-to)223-230
Number of pages8
JournalExperimental Parasitology
Volume84
Issue number2
DOIs
StatePublished - Nov 1996
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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