Plasmodium hemozoin formation mediated by histidine-rich proteins

David J. Sullivan, Ilya Y. Gluzman, Daniel E. Goldberg

Research output: Contribution to journalArticlepeer-review

Abstract

The digestive vacuole of Plasmodium falciparum is the site of hemoglobin degradation, heme polymerization into crystalline hemozoin, and antimalarial drug accumulation. Antibodies identified histidine-rich protein II (HRP II) in purified digestive vacuoles. Recombinant or native HRP II promoted the formation of hemozoin, and chloroquine inhibited the reaction. The related HRP III also polymerized heme, and an additional HRP was identified in vacuoles. It is proposed that after secretion by the parasite into the host erythrocyte cytosol, HRPs are brought into the acidic digestive vacuole along with hemoglobin. After hemoglobin proteolysis, HRPs bind the liberated heme and mediate hemozoin formation.

Original languageEnglish (US)
Pages (from-to)219-222
Number of pages4
JournalScience
Volume271
Issue number5246
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • General

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