Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells

Diana L. van de Hoef, Isabelle Coppens, Thomas Holowka, Choukri Ben Mamoun, OraLee L. Branch, Ana Rodriguez

Research output: Contribution to journalArticle

Abstract

Malaria is characterized by cyclical fevers and high levels of inflammation, and while an early inflammatory response contributes to parasite clearance, excessive and persistent inflammation can lead to severe forms of the disease. Here, we show that Plasmodium falciparum-infected erythrocytes contain uric acid precipitates in the cytoplasm of the parasitophorous vacuole, which are released when erythrocytes rupture. Uric acid precipitates are highly inflammatory molecules that are considered a danger signal for innate immunity and are the causative agent in gout. We determined that P. falciparum-derived uric acid precipitates induce maturation of human dendritic cells, increasing the expression of cell surface co-stimulatory molecules such as CD80 and CD86, while decreasing human leukocyte antigen-DR expression. In accordance with this, uric acid accounts for a significant proportion of the total stimulatory activity induced by parasite-infected erythrocytes. Moreover, the identification of uric acid precipitates in P. falciparum- and P. vivax-infected erythrocytes obtained directly from malaria patients underscores the in vivo and clinical relevance of our findings. Altogether, our data implicate uric acid precipitates as a potentially important contributor to the innate immune response to Plasmodium infection and may provide a novel target for adjunct therapies.

Original languageEnglish (US)
Article numbere55584
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 6 2013

Fingerprint

Plasmodium falciparum
uric acid
dendritic cells
Uric Acid
Dendritic Cells
Precipitates
erythrocytes
Erythrocytes
Malaria
inflammation
Innate Immunity
malaria
Parasites
gout
Inflammation
parasites
Molecules
Gout
Plasmodium
HLA Antigens

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

van de Hoef, D. L., Coppens, I., Holowka, T., Ben Mamoun, C., Branch, O. L., & Rodriguez, A. (2013). Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells. PLoS One, 8(2), [e55584]. https://doi.org/10.1371/journal.pone.0055584

Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells. / van de Hoef, Diana L.; Coppens, Isabelle; Holowka, Thomas; Ben Mamoun, Choukri; Branch, OraLee L.; Rodriguez, Ana.

In: PLoS One, Vol. 8, No. 2, e55584, 06.02.2013.

Research output: Contribution to journalArticle

van de Hoef, DL, Coppens, I, Holowka, T, Ben Mamoun, C, Branch, OL & Rodriguez, A 2013, 'Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells', PLoS One, vol. 8, no. 2, e55584. https://doi.org/10.1371/journal.pone.0055584
van de Hoef, Diana L. ; Coppens, Isabelle ; Holowka, Thomas ; Ben Mamoun, Choukri ; Branch, OraLee L. ; Rodriguez, Ana. / Plasmodium falciparum-Derived Uric Acid Precipitates Induce Maturation of Dendritic Cells. In: PLoS One. 2013 ; Vol. 8, No. 2.
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