Plasmids in Salmonella typhi in Lima, Peru, 1987-1988: Epidemiology and lack of association with severity of illness or clinical complications

M. J. Finch, A. Franco, E. Gotuzzo, C. Carrillo, L. Benavente, S. S. Wasserman, M. M. Levine, J. G. Morris

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A prospective study was conducted to better characterize the epidemiology of plasmid-bearing strains of Salmonella typhi in an endemic area of Lima, Peru, and to determine if plasmids were associated with specific manifestations of typhoid fever. Of 228 S. typhi isolated from patients at Cayetano Heredia University Hospital in Lima during 1987-1988, 76 (33%) contained plasmids. Ten different plasmid profiles were identified, with ten distinct plasmids present within these profiles. There was significant temporal clustering of isolates having common plasmid profiles. Two plasmids (both from the same isolate) carried antibiotic resistance genes. Two cryptic plasmids with approximate sizes of 55 and 65 kilobases (kb) appeared to be closely related, based on restriction endonuclease digestions and Southern blot analysis. An ampicillin resistance plasmid from a 1989 patient isolate differed by only a single restriction fragment from the cryptic 65-kb plasmid. No association was found between any plasmid or plasmid profile and severity or clinical manifestations of disease.

    Original languageEnglish (US)
    Pages (from-to)390-396
    Number of pages7
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume47
    Issue number3
    DOIs
    StatePublished - 1992

    ASJC Scopus subject areas

    • Parasitology
    • Virology
    • Infectious Diseases

    Fingerprint

    Dive into the research topics of 'Plasmids in Salmonella typhi in Lima, Peru, 1987-1988: Epidemiology and lack of association with severity of illness or clinical complications'. Together they form a unique fingerprint.

    Cite this