Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli

P. Echeverria, J. Seriwatana, D. N. Taylor, S. Changchawalit, C. J. Smyth, J. Twohig, B. Rowe

Research output: Contribution to journalArticle

Abstract

Colonization factor antigens I and II (CFA/I and CFA/II) are important in the pathogenesis of diarrhea in humans caused by some enterotoxigenic Escherichia coli (ETEC). Plasmid DNA from 16 CFA/I+ and five CFA/II+ ETEC were examined by Southern blot analysis with enterotoxin gene probes and were compared with plasmid DNA from derivatives of the same ETEC that had lost the ability to produce these colonization factors. Among the 16 CFA/I+ ETEC strains, the loss of CFA/I was accompanied by the loss of a plasmid of between 34 and 68 megadaltons (MDa) coding for heat-stable enterotoxin A2 (ST-A2) in 12 strains, by the loss of a 60-MDa plasmid coding for heat-labile enterotoxin (LT) and ST-A2 in one strain, or by deletions of a segment of DNA encoding for ST-A2 in three strains. Among five CFA/II+ ETEC strains, the loss of CFA/II was associated with the loss of a plasmid of 75 MDa coding for LT and ST-A2 in three strains, with the loss of genes coding for LT and ST-A2 from a 68-MDa plasmid in one strain, or with no discernible loss of a plasmid or DNA sequences coding for enterotoxins in the remaining strain. The loss of CFA/I and CFA/II production was associated with the loss of DNA sequences encoding for ST-A2 in 20 of 21 ETEC examined.

Original languageEnglish (US)
Pages (from-to)626-630
Number of pages5
JournalInfection and Immunity
Volume51
Issue number2
StatePublished - 1986
Externally publishedYes

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Enterotoxins
varespladib methyl
Enterotoxigenic Escherichia coli
Plasmids
Hot Temperature
Escherichia coli
DNA
colonization factor antigens
Southern Blotting
Genes
Diarrhea

ASJC Scopus subject areas

  • Immunology

Cite this

Echeverria, P., Seriwatana, J., Taylor, D. N., Changchawalit, S., Smyth, C. J., Twohig, J., & Rowe, B. (1986). Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli. Infection and Immunity, 51(2), 626-630.

Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli. / Echeverria, P.; Seriwatana, J.; Taylor, D. N.; Changchawalit, S.; Smyth, C. J.; Twohig, J.; Rowe, B.

In: Infection and Immunity, Vol. 51, No. 2, 1986, p. 626-630.

Research output: Contribution to journalArticle

Echeverria, P, Seriwatana, J, Taylor, DN, Changchawalit, S, Smyth, CJ, Twohig, J & Rowe, B 1986, 'Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli', Infection and Immunity, vol. 51, no. 2, pp. 626-630.
Echeverria P, Seriwatana J, Taylor DN, Changchawalit S, Smyth CJ, Twohig J et al. Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli. Infection and Immunity. 1986;51(2):626-630.
Echeverria, P. ; Seriwatana, J. ; Taylor, D. N. ; Changchawalit, S. ; Smyth, C. J. ; Twohig, J. ; Rowe, B. / Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli. In: Infection and Immunity. 1986 ; Vol. 51, No. 2. pp. 626-630.
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AB - Colonization factor antigens I and II (CFA/I and CFA/II) are important in the pathogenesis of diarrhea in humans caused by some enterotoxigenic Escherichia coli (ETEC). Plasmid DNA from 16 CFA/I+ and five CFA/II+ ETEC were examined by Southern blot analysis with enterotoxin gene probes and were compared with plasmid DNA from derivatives of the same ETEC that had lost the ability to produce these colonization factors. Among the 16 CFA/I+ ETEC strains, the loss of CFA/I was accompanied by the loss of a plasmid of between 34 and 68 megadaltons (MDa) coding for heat-stable enterotoxin A2 (ST-A2) in 12 strains, by the loss of a 60-MDa plasmid coding for heat-labile enterotoxin (LT) and ST-A2 in one strain, or by deletions of a segment of DNA encoding for ST-A2 in three strains. Among five CFA/II+ ETEC strains, the loss of CFA/II was associated with the loss of a plasmid of 75 MDa coding for LT and ST-A2 in three strains, with the loss of genes coding for LT and ST-A2 from a 68-MDa plasmid in one strain, or with no discernible loss of a plasmid or DNA sequences coding for enterotoxins in the remaining strain. The loss of CFA/I and CFA/II production was associated with the loss of DNA sequences encoding for ST-A2 in 20 of 21 ETEC examined.

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