Plasmid-chromosome shuffling for non-deletion alleles in yeast

Zhiwei Huang, Richard S. Sucgang, Yu Yi Lin, Xiaomin Shi, Jef D. Boeke, Xuewen Pan

Research output: Contribution to journalArticlepeer-review

Abstract

Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.

Original languageEnglish (US)
Pages (from-to)167-169
Number of pages3
JournalNature Methods
Volume5
Issue number2
DOIs
StatePublished - Feb 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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