Plasma neopterin level as a marker of peripheral immune activation in amnestic mild cognitive impairment and Alzheimer's disease

Daniel C. Parker, Michelle M. Mielke, Qilu Yu, Paul B Rosenberg, Alka Jain, Constantine G Lyketsos, Neal S Fedarko, Esther Oh

Research output: Contribution to journalArticle

Abstract

Objective Alterations of the immune system play important roles in Alzheimer's disease (AD). The primary purpose of this study was to compare the plasma levels of neopterin, a marker of cellular immune activity, in amnestic mild cognitive impairment (aMCI), early (mild to moderate) AD, and cognitively normal controls. In addition, the correlation of plasma neopterin with interferon-gamma (IFN-γ) and interleukin-6 (IL-6) was also examined. Methods Plasma samples from patients with mild-to-moderate AD (N = 34), aMCI (N = 27), and cognitively normal controls (N = 30) were obtained from the Johns Hopkins Alzheimer's Disease Research Center. Plasma neopterin, IFN-γ, and IL-6 levels were measured using commercially available ELISAs. Multiple linear regression was performed to study differences in the baseline neopterin levels between normal, aMCI, and AD patients. Pearson correlation coefficients were estimated for neopterin and IFN-γ and IL-6 levels. All analyses were conducted using SAS (SAS Institute, Inc., Cary, NC) and GraphPad Prism version 5.00 for Window (GraphPad Software, San Diego, CA, USA). Results AD subjects had significantly higher neopterin values compared with aMCI (β = 0.202, p = 0.004) and normal (β = 0.263, p = 0.0004) subjects. There was no statistically significant difference between normal and aMCI subjects. Significant associations between neopterin and IFN-γ (r = 0.41, p <0.0001) and IL-6 (r = 0.35, p = 0.0006) levels were found. Conclusions Our study demonstrates that peripheral immune response may be stronger in later stages of AD pathophysiology, when dementia has developed.

Original languageEnglish (US)
Pages (from-to)149-154
Number of pages6
JournalInternational Journal of Geriatric Psychiatry
Volume28
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Neopterin
Alzheimer Disease
Biomarkers
Interferon-gamma
Interleukin-6
Cognitive Dysfunction
Dementia
Immune System
Linear Models
Software
Enzyme-Linked Immunosorbent Assay
Research

Keywords

  • Alzheimer's disease
  • amnestic mild cognitive impairment
  • inflammation
  • neopterin

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Psychiatry and Mental health

Cite this

@article{d309035e00ad4fee8e450925fa5a0cae,
title = "Plasma neopterin level as a marker of peripheral immune activation in amnestic mild cognitive impairment and Alzheimer's disease",
abstract = "Objective Alterations of the immune system play important roles in Alzheimer's disease (AD). The primary purpose of this study was to compare the plasma levels of neopterin, a marker of cellular immune activity, in amnestic mild cognitive impairment (aMCI), early (mild to moderate) AD, and cognitively normal controls. In addition, the correlation of plasma neopterin with interferon-gamma (IFN-γ) and interleukin-6 (IL-6) was also examined. Methods Plasma samples from patients with mild-to-moderate AD (N = 34), aMCI (N = 27), and cognitively normal controls (N = 30) were obtained from the Johns Hopkins Alzheimer's Disease Research Center. Plasma neopterin, IFN-γ, and IL-6 levels were measured using commercially available ELISAs. Multiple linear regression was performed to study differences in the baseline neopterin levels between normal, aMCI, and AD patients. Pearson correlation coefficients were estimated for neopterin and IFN-γ and IL-6 levels. All analyses were conducted using SAS (SAS Institute, Inc., Cary, NC) and GraphPad Prism version 5.00 for Window (GraphPad Software, San Diego, CA, USA). Results AD subjects had significantly higher neopterin values compared with aMCI (β = 0.202, p = 0.004) and normal (β = 0.263, p = 0.0004) subjects. There was no statistically significant difference between normal and aMCI subjects. Significant associations between neopterin and IFN-γ (r = 0.41, p <0.0001) and IL-6 (r = 0.35, p = 0.0006) levels were found. Conclusions Our study demonstrates that peripheral immune response may be stronger in later stages of AD pathophysiology, when dementia has developed.",
keywords = "Alzheimer's disease, amnestic mild cognitive impairment, inflammation, neopterin",
author = "Parker, {Daniel C.} and Mielke, {Michelle M.} and Qilu Yu and Rosenberg, {Paul B} and Alka Jain and Lyketsos, {Constantine G} and Fedarko, {Neal S} and Esther Oh",
year = "2013",
month = "2",
doi = "10.1002/gps.3802",
language = "English (US)",
volume = "28",
pages = "149--154",
journal = "International Journal of Geriatric Psychiatry",
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T1 - Plasma neopterin level as a marker of peripheral immune activation in amnestic mild cognitive impairment and Alzheimer's disease

AU - Parker, Daniel C.

AU - Mielke, Michelle M.

AU - Yu, Qilu

AU - Rosenberg, Paul B

AU - Jain, Alka

AU - Lyketsos, Constantine G

AU - Fedarko, Neal S

AU - Oh, Esther

PY - 2013/2

Y1 - 2013/2

N2 - Objective Alterations of the immune system play important roles in Alzheimer's disease (AD). The primary purpose of this study was to compare the plasma levels of neopterin, a marker of cellular immune activity, in amnestic mild cognitive impairment (aMCI), early (mild to moderate) AD, and cognitively normal controls. In addition, the correlation of plasma neopterin with interferon-gamma (IFN-γ) and interleukin-6 (IL-6) was also examined. Methods Plasma samples from patients with mild-to-moderate AD (N = 34), aMCI (N = 27), and cognitively normal controls (N = 30) were obtained from the Johns Hopkins Alzheimer's Disease Research Center. Plasma neopterin, IFN-γ, and IL-6 levels were measured using commercially available ELISAs. Multiple linear regression was performed to study differences in the baseline neopterin levels between normal, aMCI, and AD patients. Pearson correlation coefficients were estimated for neopterin and IFN-γ and IL-6 levels. All analyses were conducted using SAS (SAS Institute, Inc., Cary, NC) and GraphPad Prism version 5.00 for Window (GraphPad Software, San Diego, CA, USA). Results AD subjects had significantly higher neopterin values compared with aMCI (β = 0.202, p = 0.004) and normal (β = 0.263, p = 0.0004) subjects. There was no statistically significant difference between normal and aMCI subjects. Significant associations between neopterin and IFN-γ (r = 0.41, p <0.0001) and IL-6 (r = 0.35, p = 0.0006) levels were found. Conclusions Our study demonstrates that peripheral immune response may be stronger in later stages of AD pathophysiology, when dementia has developed.

AB - Objective Alterations of the immune system play important roles in Alzheimer's disease (AD). The primary purpose of this study was to compare the plasma levels of neopterin, a marker of cellular immune activity, in amnestic mild cognitive impairment (aMCI), early (mild to moderate) AD, and cognitively normal controls. In addition, the correlation of plasma neopterin with interferon-gamma (IFN-γ) and interleukin-6 (IL-6) was also examined. Methods Plasma samples from patients with mild-to-moderate AD (N = 34), aMCI (N = 27), and cognitively normal controls (N = 30) were obtained from the Johns Hopkins Alzheimer's Disease Research Center. Plasma neopterin, IFN-γ, and IL-6 levels were measured using commercially available ELISAs. Multiple linear regression was performed to study differences in the baseline neopterin levels between normal, aMCI, and AD patients. Pearson correlation coefficients were estimated for neopterin and IFN-γ and IL-6 levels. All analyses were conducted using SAS (SAS Institute, Inc., Cary, NC) and GraphPad Prism version 5.00 for Window (GraphPad Software, San Diego, CA, USA). Results AD subjects had significantly higher neopterin values compared with aMCI (β = 0.202, p = 0.004) and normal (β = 0.263, p = 0.0004) subjects. There was no statistically significant difference between normal and aMCI subjects. Significant associations between neopterin and IFN-γ (r = 0.41, p <0.0001) and IL-6 (r = 0.35, p = 0.0006) levels were found. Conclusions Our study demonstrates that peripheral immune response may be stronger in later stages of AD pathophysiology, when dementia has developed.

KW - Alzheimer's disease

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KW - inflammation

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