Plasma markers of platelet activation in cystic fibrosis liver and lung disease

Kathleen B. Schwarz, Jeffrey Rosensweig, Savitri Sharma, Lawrence Jones, Michael Durant, Carol Potter, Michael R. Narkewicz

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Transforming growth factor beta 1 (TGFβ1) is a major fibrogenic cytokine, the expression of which is increased in the livers of children with cystic fibrosis liver disease (CFLD) and in the bronchoalveolar lavage fluid of patients with cystic fibrosis pulmonary disease (CFPD). The purpose of our study was to investigate the usefulness of plasma TGFβ1 as a noninvasive marker of CFLD and CFPD, or both and to investigate the contribution of platelet-derived TGFβ1 to plasma TGFβ1 by correlating the latter with platelet factor 4 (PF4). Methods: Three groups of patients with cystic fibrosis were studied: 1) those with CFLD, 2) those with CF and no liver disease (CFNLD), and 3) those with CFPD. Controls were healthy adolescents and adults. Plasma TGFβ1 was assayed using the R and D Quantikine quantitative sandwich enzyme immunoassay technique and PF4 (American Bioproducts ELISA kit). Results: Plasma TGFβ1 was markedly increased in CFPD (15 ± 3 ng/mL) versus healthy adults (1 ± 0 ng/mL; P < 0.004. The PF4 values followed a similar pattern: 105 ± 8 ng/mL in CFPD versus 12 ± 4 ng/mL (P < 0.0001). Plasma TGFβ1 values in CFLD did not differ from CFNLD patients of comparable age nor from values for healthy adolescents. Plasma TGFβ1 values were strongly correlated with values for PF4: r = 0.543, P < 0.0001. Conclusions: Plasma TGFβ1 is not a useful marker of CFLD. The increased plasma TGFβ1 and PF4 in CFPD patients are of interest both as possible noninvasive markers of pulmonary fibrosis and because the increased values suggest that platelet activation may play a pathogenetic role in CFPD.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalJournal of pediatric gastroenterology and nutrition
Volume37
Issue number2
DOIs
StatePublished - Aug 2003

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

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