Plasma exchange does not improve survival in a canine model of human septic shock

C. Natanson, W. D. Hoffman, L. A. Koev, D. P. Dolan, S. M. Banks, J. Bacher, R. L. Danner, H. G. Klein, J. E. Parrillo

Research output: Contribution to journalArticle

Abstract

Whether plasma exchange would improve survival in antibiotic-treated canines with septic shock was investigated. Escherichia coli O86H8 (1.4 x 1010) was surgically implanted as an intraperitoneal clot in 18 two-year- old (10-12 kg) purpose-bred beagles. Beginning 4 hours after surgery, all animals received cefoxitin and gentamicin for 5 days. Three treatment groups were defined: 1) a no apheresis, or control group, (n = 6); 2) a sham apheresis group, whose whole blood plasma was removed, separated, and then transfused (n = 6); and 3) a plasma exchange group from whom blood and plasma were removed and separated, to whom the blood was returned, and in whom infected plasma was replaced with compatible fresh-frozen canine plasma (n = 6). For the sham apheresis and plasma exchange groups, a commercial blood cell processor was used to separate 1.5 blood volumes of plasma at 5 and 24 hours after surgery. Serial radionuclide left ventricular ejection fractions and femoral and pulmonary arterial catheter hemodynamics were measured simultaneously in awake animals. All six animals in the plasma exchange group died. In both the sham and control groups, only one of six animals survived. Survival times were ordered (median in hours) (control [372 h] > sham apheresis [48 h] > plasma exchange [24 h] [p sham apheresis > control; p

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalTransfusion
Volume33
Issue number3
StatePublished - 1993
Externally publishedYes

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Blood Component Removal
Plasma Exchange
Septic Shock
Canidae
Survival
Cefoxitin
Control Groups
Plasma Volume
Thigh
Gentamicins
Radioisotopes
Stroke Volume
Blood Cells
Catheters
Hemodynamics
Escherichia coli
Anti-Bacterial Agents
Lung

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Natanson, C., Hoffman, W. D., Koev, L. A., Dolan, D. P., Banks, S. M., Bacher, J., ... Parrillo, J. E. (1993). Plasma exchange does not improve survival in a canine model of human septic shock. Transfusion, 33(3), 243-248.

Plasma exchange does not improve survival in a canine model of human septic shock. / Natanson, C.; Hoffman, W. D.; Koev, L. A.; Dolan, D. P.; Banks, S. M.; Bacher, J.; Danner, R. L.; Klein, H. G.; Parrillo, J. E.

In: Transfusion, Vol. 33, No. 3, 1993, p. 243-248.

Research output: Contribution to journalArticle

Natanson, C, Hoffman, WD, Koev, LA, Dolan, DP, Banks, SM, Bacher, J, Danner, RL, Klein, HG & Parrillo, JE 1993, 'Plasma exchange does not improve survival in a canine model of human septic shock', Transfusion, vol. 33, no. 3, pp. 243-248.
Natanson C, Hoffman WD, Koev LA, Dolan DP, Banks SM, Bacher J et al. Plasma exchange does not improve survival in a canine model of human septic shock. Transfusion. 1993;33(3):243-248.
Natanson, C. ; Hoffman, W. D. ; Koev, L. A. ; Dolan, D. P. ; Banks, S. M. ; Bacher, J. ; Danner, R. L. ; Klein, H. G. ; Parrillo, J. E. / Plasma exchange does not improve survival in a canine model of human septic shock. In: Transfusion. 1993 ; Vol. 33, No. 3. pp. 243-248.
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