Plasma butylcholinesterase activity and cocaine half-life differ significantly in rhesus and squirrel monkeys

G. N. Carmona, I. Baum, C. W. Schindler, S. R. Goldberg, R. Jufer, E. Cone, E. Slaughter, G. W. Belendiuk, David A. Gorelick

Research output: Contribution to journalArticle

Abstract

In vitro studies have implicated butyrylcholinesterase (BChE, E.C.3.1.1.8) as the major enzyme for metabolizing cocaine in humans, but little is known about endogenous BChE activity in monkeys and other animals often used in preclinical studies of cocaine. We compared BChE activity in 18 rhesus and 11 squirrel monkeys, using the colorimetric method of Ellman with butyrylthiocholine as substrate, and in vitro cocaine half-life in pooled plasma samples measuring cocaine concentrations over 60 minutes by CC-MS. Rhesus monkeys had a significantly higher plasma BChE activity than squirrel monkeys (8.2 ± 0.5 U/L vs. 2.8 ± 0.5 U/L), and a three-fold shorter in vitro cocaine half-life (20.1 min vs. 60.2 min). BChE activity in rhesus monkeys was comparative to the activity reported in humans. There was no significant influence of age, weight, or prior cocaine exposure. These results indicate that BChE level can vary between species of non-human primates, a factor that should be taken into account when studying drugs such as cocaine which are metabolized by BChE.

Original languageEnglish (US)
Pages (from-to)939-943
Number of pages5
JournalLife Sciences
Volume59
Issue number11
DOIs
StatePublished - Aug 9 1996
Externally publishedYes

Fingerprint

Saimiri
Macaca mulatta
Cocaine
Half-Life
Plasmas
Butyrylthiocholine
Butyrylcholinesterase
Sciuridae
Primates
Haplorhini
Animals
Weights and Measures
Substrates
Enzymes
Pharmaceutical Preparations

Keywords

  • Butyrylcholinesterase
  • Cocaine
  • Rhesus monkeys
  • Squirrel monkey

ASJC Scopus subject areas

  • Pharmacology

Cite this

Carmona, G. N., Baum, I., Schindler, C. W., Goldberg, S. R., Jufer, R., Cone, E., ... Gorelick, D. A. (1996). Plasma butylcholinesterase activity and cocaine half-life differ significantly in rhesus and squirrel monkeys. Life Sciences, 59(11), 939-943. https://doi.org/10.1016/0024-3205(96)00392-X

Plasma butylcholinesterase activity and cocaine half-life differ significantly in rhesus and squirrel monkeys. / Carmona, G. N.; Baum, I.; Schindler, C. W.; Goldberg, S. R.; Jufer, R.; Cone, E.; Slaughter, E.; Belendiuk, G. W.; Gorelick, David A.

In: Life Sciences, Vol. 59, No. 11, 09.08.1996, p. 939-943.

Research output: Contribution to journalArticle

Carmona, GN, Baum, I, Schindler, CW, Goldberg, SR, Jufer, R, Cone, E, Slaughter, E, Belendiuk, GW & Gorelick, DA 1996, 'Plasma butylcholinesterase activity and cocaine half-life differ significantly in rhesus and squirrel monkeys', Life Sciences, vol. 59, no. 11, pp. 939-943. https://doi.org/10.1016/0024-3205(96)00392-X
Carmona, G. N. ; Baum, I. ; Schindler, C. W. ; Goldberg, S. R. ; Jufer, R. ; Cone, E. ; Slaughter, E. ; Belendiuk, G. W. ; Gorelick, David A. / Plasma butylcholinesterase activity and cocaine half-life differ significantly in rhesus and squirrel monkeys. In: Life Sciences. 1996 ; Vol. 59, No. 11. pp. 939-943.
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