TY - JOUR
T1 - Plasma 5-aminolevulinic acid concentration and lead exposure in children
AU - Sithisarankul, Pornchai
AU - Cadorette, Maureen
AU - Davoli, Cecilia T.
AU - Serwint, Janet R.
AU - Chisolm, James J.
AU - Strickland, Paul T.
N1 - Funding Information:
The authors thank Dr. Katsumaro Tomokuni for advice on derivatization methods and the clinical staffs at the Kennedy Krieger Institute and the Harriet Lane Clinic for their assistance in patient recruitment and sample collection. This research was sponsored in part by DHHS Grants ES03819, ES35362, and CCT310419. This study was conducted in accordance with guidelines for the protection of human subjects. The study design and consent procedures were approved by the Joint Committee on Clinical Investigation (Johns Hopkins University School of Medicine and Johns Hopkins Hospital).
PY - 1999/1
Y1 - 1999/1
N2 - The relationship between concentration of 5-aminolevulinic acid in plasma (ALAP) and other biomarkers of lead exposure and effect was investigated in lead-exposed children. We measured ALAP by chemical derivatization and high-performance liquid chromatography with fluorescence detection. The study population consisted of 103 children: 78 from a referral clinic for children with lead poisoning and 25 from a general pediatric clinic. Blood lead concentration (PbB), age, and were higher in lead clinic subjects than in general clinic subjects. ALAP was significantly correlated with PbB (Spearman r = 0.38; P= 0,0007) and free erythrocyte protoporphyrin concentration (r = 0.41, P= 0.0002) in lead clinic subjects. PbB was a significant predictor of ALAP (P=0,0001) by multiple linear regression in all subjects. The average PbB in the 3- to 12-month period prior to blood collection correlated with ALAP to the same degree that current PbB correlated with ALAP. Possible associations between ALAP and adverse health outcomes, particularly neurobehavioral effects, should be investigated in children to assess the predictive value of ALAP for these endpoints.
AB - The relationship between concentration of 5-aminolevulinic acid in plasma (ALAP) and other biomarkers of lead exposure and effect was investigated in lead-exposed children. We measured ALAP by chemical derivatization and high-performance liquid chromatography with fluorescence detection. The study population consisted of 103 children: 78 from a referral clinic for children with lead poisoning and 25 from a general pediatric clinic. Blood lead concentration (PbB), age, and were higher in lead clinic subjects than in general clinic subjects. ALAP was significantly correlated with PbB (Spearman r = 0.38; P= 0,0007) and free erythrocyte protoporphyrin concentration (r = 0.41, P= 0.0002) in lead clinic subjects. PbB was a significant predictor of ALAP (P=0,0001) by multiple linear regression in all subjects. The average PbB in the 3- to 12-month period prior to blood collection correlated with ALAP to the same degree that current PbB correlated with ALAP. Possible associations between ALAP and adverse health outcomes, particularly neurobehavioral effects, should be investigated in children to assess the predictive value of ALAP for these endpoints.
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U2 - 10.1006/enrs.1998.3883
DO - 10.1006/enrs.1998.3883
M3 - Article
C2 - 9931226
AN - SCOPUS:0032929409
SN - 0013-9351
VL - 80
SP - 41
EP - 49
JO - Environmental research
JF - Environmental research
IS - 1
ER -