Plaque formation and purification of BK virus in cultured human urinary cells

George Henry Sack, J. S. Felix, A. A. Lanahan

Research output: Contribution to journalArticle

Abstract

Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.

Original languageEnglish (US)
Pages (from-to)185-189
Number of pages5
JournalJournal of General Virology
Volume50
Issue number1
StatePublished - 1980

Fingerprint

BK Virus
Cultural Characteristics
Viruses
Epithelial Cells
DNA
Enzymes
Growth

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Plaque formation and purification of BK virus in cultured human urinary cells. / Sack, George Henry; Felix, J. S.; Lanahan, A. A.

In: Journal of General Virology, Vol. 50, No. 1, 1980, p. 185-189.

Research output: Contribution to journalArticle

@article{8c19c9be86a84a0a8a1504508ac6d03f,
title = "Plaque formation and purification of BK virus in cultured human urinary cells",
abstract = "Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.",
author = "Sack, {George Henry} and Felix, {J. S.} and Lanahan, {A. A.}",
year = "1980",
language = "English (US)",
volume = "50",
pages = "185--189",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "1",

}

TY - JOUR

T1 - Plaque formation and purification of BK virus in cultured human urinary cells

AU - Sack, George Henry

AU - Felix, J. S.

AU - Lanahan, A. A.

PY - 1980

Y1 - 1980

N2 - Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.

AB - Primary human urinary cells support growth and plaque formation by papovavirus BK, permitting plaque purification of the virus. After plaquing, the virus forms uniform plaques and its DNA has a homogeneous restriction enzyme fragment profile. Support of BK replication as well as morphological and cultural characteristics suggest an epithelial origin for the cells.

UR - http://www.scopus.com/inward/record.url?scp=0019128643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019128643&partnerID=8YFLogxK

M3 - Article

C2 - 6255084

AN - SCOPUS:0019128643

VL - 50

SP - 185

EP - 189

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 1

ER -