Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine

Antje Heese-Peck, Robert N Cole, Olga N. Borkhsenious, Gerald Warren Hart, Natasha V. Raikhel

Research output: Contribution to journalArticle

Abstract

Only a few nuclear pore complex (NPC) proteins, mainly in vertebrates and yeast but none in plants, have been well characterized. As an initial step to identify plant NPC proteins, we examined whether NPC proteins from tobacco are modified by N-acetylglucosamine (GlcNAc). Using wheat germ agglutinin, a lectin that binds specifically to GlcNAc in plants, specific labeling was often found associated with or adjacent to NPCs. Nuclear proteins containing GlcNAc can be partially extracted by 0.5 M salt, as shown by a wheat germ agglutinin blot assay, and at least eight extracted proteins were modified by terminal GlcNAc, as determined by in vitro galactosyltransferase assays. Sugar analysis indicated that the plant glycans with terminal GlcNAc differ from the single O-linked GlcNAc of vertebrate NPC proteins in that they consist of oligosaccharides that are larger in size than five GlcNAc residues. Most of these appear to be bound to proteins via a hydroxyl group. This novel oligosaccharide modification may convey properties to the plant NPC that are different from those of vertebrate NPCs.

Original languageEnglish (US)
Pages (from-to)1459-1471
Number of pages13
JournalPlant Cell
Volume7
Issue number9
StatePublished - Sep 1995
Externally publishedYes

Fingerprint

Nuclear Pore Complex Proteins
nucleoporins
N-acetylglucosamine
Acetylglucosamine
Oligosaccharides
oligosaccharides
Wheat Germ Agglutinins
Vertebrates
wheat germ
vertebrates
agglutinins
Assays
Galactosyltransferases
Tobacco
galactosyltransferases
Nuclear Pore
Nuclear Proteins
Lectins
Sugars
Hydroxyl Radical

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Heese-Peck, A., Cole, R. N., Borkhsenious, O. N., Hart, G. W., & Raikhel, N. V. (1995). Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine. Plant Cell, 7(9), 1459-1471.

Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine. / Heese-Peck, Antje; Cole, Robert N; Borkhsenious, Olga N.; Hart, Gerald Warren; Raikhel, Natasha V.

In: Plant Cell, Vol. 7, No. 9, 09.1995, p. 1459-1471.

Research output: Contribution to journalArticle

Heese-Peck, A, Cole, RN, Borkhsenious, ON, Hart, GW & Raikhel, NV 1995, 'Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine', Plant Cell, vol. 7, no. 9, pp. 1459-1471.
Heese-Peck, Antje ; Cole, Robert N ; Borkhsenious, Olga N. ; Hart, Gerald Warren ; Raikhel, Natasha V. / Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine. In: Plant Cell. 1995 ; Vol. 7, No. 9. pp. 1459-1471.
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