TY - JOUR
T1 - Placental vitamin D receptor (VDR) expression is related to neonatal vitamin D status, placental calcium transfer, and fetal bone length in pregnant adolescents
AU - Young, Bridget E.
AU - Cooper, Elizabeth M.
AU - McIntyre, Allison W.
AU - Kent, Tera
AU - Witter, Frank
AU - Harris, Z. Leah
AU - O'Brien, Kimberly O.
PY - 2014/5
Y1 - 2014/5
N2 - The purpose of the study was to identify determinants of placental vitamin D receptor (VDR) expression and placental calcium (Ca) transfer among pregnant adolescents. Placental tissue was obtained in 94 adolescents (≥18 yr) at term. In 12 of these teens, stable Ca isotopes were given intravenously ( 42Ca) and orally (44Ca) early in labor. Placental VDR expression was assessed via Western blot and validated by RTPCR. Maternal-to-fetal Ca transfer was calculated as the enrichment in cord blood at delivery relative to maternal serum enrichment 2 h postdosing. Isotopic study outcomes were examined in relation to fetal long bone length, placental VDR, serum 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH) 2D], and parathyroid hormone (PTH) in maternal circulation and cord blood at delivery. Placental VDR expression was inversely associated with neonatal 25(OH)D (P=0.012) and positively with neonatal 1,25(OH)2D (P=0.006). Placental VDR was a positive predictor of fetal femur length Z score (P=0.018; R2=0.06) and was positively correlated with maternal-to-fetal transfer of intravenous 42Ca (P=0.004; R2=0.62). The fetus may regulate placental VDR expression given the significant associations with neonatal vitamin D metabolites. The association between placental VDR and fetal long bone length may indicate a role for VDR in fetal bone development, potentially by mediating transplacental Ca transfer.
AB - The purpose of the study was to identify determinants of placental vitamin D receptor (VDR) expression and placental calcium (Ca) transfer among pregnant adolescents. Placental tissue was obtained in 94 adolescents (≥18 yr) at term. In 12 of these teens, stable Ca isotopes were given intravenously ( 42Ca) and orally (44Ca) early in labor. Placental VDR expression was assessed via Western blot and validated by RTPCR. Maternal-to-fetal Ca transfer was calculated as the enrichment in cord blood at delivery relative to maternal serum enrichment 2 h postdosing. Isotopic study outcomes were examined in relation to fetal long bone length, placental VDR, serum 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH) 2D], and parathyroid hormone (PTH) in maternal circulation and cord blood at delivery. Placental VDR expression was inversely associated with neonatal 25(OH)D (P=0.012) and positively with neonatal 1,25(OH)2D (P=0.006). Placental VDR was a positive predictor of fetal femur length Z score (P=0.018; R2=0.06) and was positively correlated with maternal-to-fetal transfer of intravenous 42Ca (P=0.004; R2=0.62). The fetus may regulate placental VDR expression given the significant associations with neonatal vitamin D metabolites. The association between placental VDR and fetal long bone length may indicate a role for VDR in fetal bone development, potentially by mediating transplacental Ca transfer.
KW - 1,25(OH)2D
KW - 25(OH)D
KW - Calcidiol
KW - Gestation
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UR - http://www.scopus.com/inward/citedby.url?scp=84901049782&partnerID=8YFLogxK
U2 - 10.1096/fj.13-246736
DO - 10.1096/fj.13-246736
M3 - Article
C2 - 24558197
AN - SCOPUS:84901049782
SN - 0892-6638
VL - 28
SP - 2029
EP - 2037
JO - FASEB Journal
JF - FASEB Journal
IS - 5
ER -