Placental vitamin D receptor (VDR) expression is related to neonatal vitamin D status, placental calcium transfer, and fetal bone length in pregnant adolescents

Bridget E. Young, Elizabeth M. Cooper, Allison W. McIntyre, Tera Kent, Frank R Witter, Z. Leah Harris, Kimberly O. O'Brien

Research output: Contribution to journalArticle

Abstract

The purpose of the study was to identify determinants of placental vitamin D receptor (VDR) expression and placental calcium (Ca) transfer among pregnant adolescents. Placental tissue was obtained in 94 adolescents (≥18 yr) at term. In 12 of these teens, stable Ca isotopes were given intravenously ( 42Ca) and orally (44Ca) early in labor. Placental VDR expression was assessed via Western blot and validated by RTPCR. Maternal-to-fetal Ca transfer was calculated as the enrichment in cord blood at delivery relative to maternal serum enrichment 2 h postdosing. Isotopic study outcomes were examined in relation to fetal long bone length, placental VDR, serum 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH) 2D], and parathyroid hormone (PTH) in maternal circulation and cord blood at delivery. Placental VDR expression was inversely associated with neonatal 25(OH)D (P=0.012) and positively with neonatal 1,25(OH)2D (P=0.006). Placental VDR was a positive predictor of fetal femur length Z score (P=0.018; R2=0.06) and was positively correlated with maternal-to-fetal transfer of intravenous 42Ca (P=0.004; R2=0.62). The fetus may regulate placental VDR expression given the significant associations with neonatal vitamin D metabolites. The association between placental VDR and fetal long bone length may indicate a role for VDR in fetal bone development, potentially by mediating transplacental Ca transfer.

Original languageEnglish (US)
Pages (from-to)2029-2037
Number of pages9
JournalFASEB Journal
Volume28
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Calcitriol Receptors
Vitamin D
Bone
Calcium
Bone and Bones
Mothers
Fetal Blood
Calcium Isotopes
Blood
Bone Development
Metabolites
Fetal Development
Parathyroid Hormone
Serum
Femur
Fetus
Western Blotting
Outcome Assessment (Health Care)
Personnel
Tissue

Keywords

  • 1,25(OH)2D
  • 25(OH)D
  • Calcidiol
  • Gestation

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Placental vitamin D receptor (VDR) expression is related to neonatal vitamin D status, placental calcium transfer, and fetal bone length in pregnant adolescents. / Young, Bridget E.; Cooper, Elizabeth M.; McIntyre, Allison W.; Kent, Tera; Witter, Frank R; Harris, Z. Leah; O'Brien, Kimberly O.

In: FASEB Journal, Vol. 28, No. 5, 2014, p. 2029-2037.

Research output: Contribution to journalArticle

Young, Bridget E. ; Cooper, Elizabeth M. ; McIntyre, Allison W. ; Kent, Tera ; Witter, Frank R ; Harris, Z. Leah ; O'Brien, Kimberly O. / Placental vitamin D receptor (VDR) expression is related to neonatal vitamin D status, placental calcium transfer, and fetal bone length in pregnant adolescents. In: FASEB Journal. 2014 ; Vol. 28, No. 5. pp. 2029-2037.
@article{6e1f42d1822b4076a58722e103ce4947,
title = "Placental vitamin D receptor (VDR) expression is related to neonatal vitamin D status, placental calcium transfer, and fetal bone length in pregnant adolescents",
abstract = "The purpose of the study was to identify determinants of placental vitamin D receptor (VDR) expression and placental calcium (Ca) transfer among pregnant adolescents. Placental tissue was obtained in 94 adolescents (≥18 yr) at term. In 12 of these teens, stable Ca isotopes were given intravenously ( 42Ca) and orally (44Ca) early in labor. Placental VDR expression was assessed via Western blot and validated by RTPCR. Maternal-to-fetal Ca transfer was calculated as the enrichment in cord blood at delivery relative to maternal serum enrichment 2 h postdosing. Isotopic study outcomes were examined in relation to fetal long bone length, placental VDR, serum 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH) 2D], and parathyroid hormone (PTH) in maternal circulation and cord blood at delivery. Placental VDR expression was inversely associated with neonatal 25(OH)D (P=0.012) and positively with neonatal 1,25(OH)2D (P=0.006). Placental VDR was a positive predictor of fetal femur length Z score (P=0.018; R2=0.06) and was positively correlated with maternal-to-fetal transfer of intravenous 42Ca (P=0.004; R2=0.62). The fetus may regulate placental VDR expression given the significant associations with neonatal vitamin D metabolites. The association between placental VDR and fetal long bone length may indicate a role for VDR in fetal bone development, potentially by mediating transplacental Ca transfer.",
keywords = "1,25(OH)2D, 25(OH)D, Calcidiol, Gestation",
author = "Young, {Bridget E.} and Cooper, {Elizabeth M.} and McIntyre, {Allison W.} and Tera Kent and Witter, {Frank R} and Harris, {Z. Leah} and O'Brien, {Kimberly O.}",
year = "2014",
doi = "10.1096/fj.13-246736",
language = "English (US)",
volume = "28",
pages = "2029--2037",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "5",

}

TY - JOUR

T1 - Placental vitamin D receptor (VDR) expression is related to neonatal vitamin D status, placental calcium transfer, and fetal bone length in pregnant adolescents

AU - Young, Bridget E.

AU - Cooper, Elizabeth M.

AU - McIntyre, Allison W.

AU - Kent, Tera

AU - Witter, Frank R

AU - Harris, Z. Leah

AU - O'Brien, Kimberly O.

PY - 2014

Y1 - 2014

N2 - The purpose of the study was to identify determinants of placental vitamin D receptor (VDR) expression and placental calcium (Ca) transfer among pregnant adolescents. Placental tissue was obtained in 94 adolescents (≥18 yr) at term. In 12 of these teens, stable Ca isotopes were given intravenously ( 42Ca) and orally (44Ca) early in labor. Placental VDR expression was assessed via Western blot and validated by RTPCR. Maternal-to-fetal Ca transfer was calculated as the enrichment in cord blood at delivery relative to maternal serum enrichment 2 h postdosing. Isotopic study outcomes were examined in relation to fetal long bone length, placental VDR, serum 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH) 2D], and parathyroid hormone (PTH) in maternal circulation and cord blood at delivery. Placental VDR expression was inversely associated with neonatal 25(OH)D (P=0.012) and positively with neonatal 1,25(OH)2D (P=0.006). Placental VDR was a positive predictor of fetal femur length Z score (P=0.018; R2=0.06) and was positively correlated with maternal-to-fetal transfer of intravenous 42Ca (P=0.004; R2=0.62). The fetus may regulate placental VDR expression given the significant associations with neonatal vitamin D metabolites. The association between placental VDR and fetal long bone length may indicate a role for VDR in fetal bone development, potentially by mediating transplacental Ca transfer.

AB - The purpose of the study was to identify determinants of placental vitamin D receptor (VDR) expression and placental calcium (Ca) transfer among pregnant adolescents. Placental tissue was obtained in 94 adolescents (≥18 yr) at term. In 12 of these teens, stable Ca isotopes were given intravenously ( 42Ca) and orally (44Ca) early in labor. Placental VDR expression was assessed via Western blot and validated by RTPCR. Maternal-to-fetal Ca transfer was calculated as the enrichment in cord blood at delivery relative to maternal serum enrichment 2 h postdosing. Isotopic study outcomes were examined in relation to fetal long bone length, placental VDR, serum 25-hydroxyvitamin D [25(OH)D], 1,25-dihydroxyvitamin D [1,25(OH) 2D], and parathyroid hormone (PTH) in maternal circulation and cord blood at delivery. Placental VDR expression was inversely associated with neonatal 25(OH)D (P=0.012) and positively with neonatal 1,25(OH)2D (P=0.006). Placental VDR was a positive predictor of fetal femur length Z score (P=0.018; R2=0.06) and was positively correlated with maternal-to-fetal transfer of intravenous 42Ca (P=0.004; R2=0.62). The fetus may regulate placental VDR expression given the significant associations with neonatal vitamin D metabolites. The association between placental VDR and fetal long bone length may indicate a role for VDR in fetal bone development, potentially by mediating transplacental Ca transfer.

KW - 1,25(OH)2D

KW - 25(OH)D

KW - Calcidiol

KW - Gestation

UR - http://www.scopus.com/inward/record.url?scp=84901049782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901049782&partnerID=8YFLogxK

U2 - 10.1096/fj.13-246736

DO - 10.1096/fj.13-246736

M3 - Article

C2 - 24558197

AN - SCOPUS:84901049782

VL - 28

SP - 2029

EP - 2037

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 5

ER -