Placental perivascular cells for human muscle regeneration

Tea Soon Park, Manuela Gavina, Chien Wen Chen, Bin Sun, Pang Ning Teng, Johnny Huard, Bridget M. Deasy, Ludovic Zimmerlin, Bruno Péault

Research output: Contribution to journalArticlepeer-review

Abstract

Perivascular multipotent mesenchymal progenitors exist in a variety of tissues, including the placenta. Here, we suggest that the abundant vasculature present in the human placenta can serve as a source of myogenic cells to regenerate skeletal muscle. Chorionic villi dissected from the mid-gestation human placenta were first transplanted intact into the gastrocnemius muscles of SCID/mdx mice, where they participated in muscle regeneration by producing myofibers expressing human dystrophin and spectrin. In vitro-cultured placental villi released rapidly adhering and migratory CD146+CD34-CD45-CD56- cells of putative perivascular origin that expressed mesenchymal stem cell markers. CD146+CD34-CD45-CD56- perivascular cells isolated and purified from the placental villi by flow cytometry were indeed highly myogenic in culture, and generated dystrophin-positive myofibers, and they promoted angiogenesis after transplantation into SCID/mdx mouse muscles. These observations confirm the existence of mesenchymal progenitor cells within the walls of human blood vessels, and suggest that the richly vascularized human placenta is an abundant source of perivascular myogenic cells able to migrate within dystrophic muscle and regenerate myofibers.

Original languageEnglish (US)
Pages (from-to)451-463
Number of pages13
JournalStem Cells and Development
Volume20
Issue number3
DOIs
StatePublished - Mar 1 2011

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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