Abstract
Dexras1 is a small GTPase and plays a central role in neuronal iron trafficking. We have shown that stimulation of glutamate receptors activates neuronal nitric oxide synthase, leading to S-nitrosylation of Dexras1 and a physiological increase in iron uptake. Here we report that Dexras1 is phosphorylated by protein kinase A (PKA) on serine 253, leading to a suppression of iron influx. These effects were directly associated with the levels of S-nitrosylated Dexras1, whereby PKA activation reduced Dexras1 S-nitrosylation in a dose dependent manner. Moreover, we found that adiponectin modulates Dexras1 via PKA. Hence these findings suggest the involvement of the PKA pathway in modulating glutamate-mediated ROS in neurons, and hint to a functional crosstalk between S-nitrosylation and phosphorylation.
Original language | English (US) |
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Article number | 37351 |
Pages (from-to) | 3212-3219 |
Number of pages | 8 |
Journal | FEBS Letters |
Volume | 589 |
Issue number | 20 |
DOIs | |
State | Published - Oct 7 2015 |
Externally published | Yes |
Keywords
- Iron
- Neuron
- Nitric oxide
- Phosphorylation
- Post-translational modification
- S-nitrosylation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology