Pirt contributes to uterine contraction-induced pain in mice

Changming Wang, Zhongli Wang, Yan Yang, Chan Zhu, Guanyi Wu, Guang Yu, Tunyu Jian, Niuniu Yang, Hao Shi, Min Tang, Qian He, Lei Lan, Qin Liu, Yun Guan, Xinzhong Dong, Jinao Duan, Zongxiang Tang

Research output: Contribution to journalArticle

Abstract

Uterine contraction-induced pain (UCP) represents a common and severe form of visceral pain. Nerve fibers that innervate uterine tissue express the transient receptor potential vanilloid channel 1 (TRPV1), which has been shown to be involved in the perception of UCP. The phosphoinositide-interacting regulator of TRP (Pirt) may act as a regulatory subunit of TRPV1. The intraperitoneal injection of oxytocin into female mice after a 6-day priming treatment with estradiol benzoate induces writhing responses, which reflect the presence of UCP. Here, we first compared writhing response between Pirt+/+ and Pirt-/- mice. Second, we examined the innervation of Pirt-expressing nerves in the uterus of Pirt-/- mice by immunofluorescence and two-photon microscopy. Third, we identified the soma of dorsal root ganglion (DRG) neurons that innerve the uterus using retrograde tracing and further characterized the neurochemical properties of these DRG neurons. Finally, we compared the calcium response of capsaicin between DRG neurons from Pirt +/+ and Pirt-/- mice. We found that the writhing responses were less intensive in Pirt-/- mice than in Pirt+/+ mice. We also observed Pirt-expressing nerve fibers in the myometrium of the uterus, and that retrograde-labeled cells were small-diameter, unmyelinated, and Pirt-positive DRG neurons. Additionally, we found that the number of capsaicin-responding neurons and the magnitude of evoked calcium response were markedly reduced in DRG neurons from Pirt-/- mice. Taken together, we speculate that Pirt plays an important role in mice uterine contraction-induced pain.

Original languageEnglish (US)
Article number57
JournalMolecular Pain
Volume11
Issue number1
DOIs
StatePublished - Sep 17 2015

Fingerprint

Uterine Contraction
Phosphatidylinositols
Pain
Spinal Ganglia
Neurons
Uterus
Transient Receptor Potential Channels
Capsaicin
Nerve Fibers
Visceral Pain
Calcium
Myometrium
Carisoprodol
Oxytocin
Intraperitoneal Injections
Photons
Fluorescent Antibody Technique
Microscopy

Keywords

  • Dorsal root ganglion
  • Pirt
  • TRPV1
  • Uterine contraction-induced pain

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Wang, C., Wang, Z., Yang, Y., Zhu, C., Wu, G., Yu, G., ... Tang, Z. (2015). Pirt contributes to uterine contraction-induced pain in mice. Molecular Pain, 11(1), [57]. https://doi.org/10.1186/s12990-015-0054-x

Pirt contributes to uterine contraction-induced pain in mice. / Wang, Changming; Wang, Zhongli; Yang, Yan; Zhu, Chan; Wu, Guanyi; Yu, Guang; Jian, Tunyu; Yang, Niuniu; Shi, Hao; Tang, Min; He, Qian; Lan, Lei; Liu, Qin; Guan, Yun; Dong, Xinzhong; Duan, Jinao; Tang, Zongxiang.

In: Molecular Pain, Vol. 11, No. 1, 57, 17.09.2015.

Research output: Contribution to journalArticle

Wang, C, Wang, Z, Yang, Y, Zhu, C, Wu, G, Yu, G, Jian, T, Yang, N, Shi, H, Tang, M, He, Q, Lan, L, Liu, Q, Guan, Y, Dong, X, Duan, J & Tang, Z 2015, 'Pirt contributes to uterine contraction-induced pain in mice', Molecular Pain, vol. 11, no. 1, 57. https://doi.org/10.1186/s12990-015-0054-x
Wang, Changming ; Wang, Zhongli ; Yang, Yan ; Zhu, Chan ; Wu, Guanyi ; Yu, Guang ; Jian, Tunyu ; Yang, Niuniu ; Shi, Hao ; Tang, Min ; He, Qian ; Lan, Lei ; Liu, Qin ; Guan, Yun ; Dong, Xinzhong ; Duan, Jinao ; Tang, Zongxiang. / Pirt contributes to uterine contraction-induced pain in mice. In: Molecular Pain. 2015 ; Vol. 11, No. 1.
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AU - Yang, Niuniu

AU - Shi, Hao

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