PIG-B: A homemade monophasic cocktail for the extraction of RNA

Kristy Weber, Mark E. Bolander, Gobinda Sarkar

Research output: Contribution to journalArticlepeer-review

Abstract

An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alochol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained by TRIzol, an expensive but widely used monophasic reagent available commercially. The complete composition and method of preparation of PIG-B is provided to aid preparation of the reagent in the laboratory.

Original languageEnglish (US)
Pages (from-to)73-77
Number of pages5
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume9
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • RNA extraction
  • RT-PCR amplification
  • Reagent

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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