Phytohemagglutinin-mediated blastomere aggregation and development of allophenic mice

Beatrice Mintz; John D. Gearhart; Agnes O. Guymont

doi:10.1016/0012-1606(73)90331-X

Phytohemagglutinin-mediated blastomere aggregation and development of allophenic mice

Beatrice Mintz, John D. Gearhart, Agnes O. Guymont

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

Abstract

Phytohemagglutinin (PHA) causes cells from two or more preimplantation-stage mouse embryos (after removal of the zona pellucida) to adhere rapidly and firmly upon a few minutes' exposure in vitro. Adhesion is successful at room temperature, and the cells remain agglutinated after transfer to room-temperature culture medium without PHA. The incubated aggregates continue their development. After surgical transfer to pseudopregnant recipients, aggregates comprising two different cellular genotypic populations can give rise to viable allophenic mice with both cellular genotypes. PHA treatment is thus a simple substitute for temperature-induced (37°C) aggregation of mouse blastomeres in vitro, and it provides a new and efficient tool for experimental studies of mammalian development.

Original language	English (US)
Pages (from-to)	195-199
Number of pages	5
Journal	Developmental Biology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(73)90331-X
State	Published - 1973
Externally published	Yes

ASJC Scopus subject areas

Developmental Biology

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title = "Phytohemagglutinin-mediated blastomere aggregation and development of allophenic mice",

abstract = "Phytohemagglutinin (PHA) causes cells from two or more preimplantation-stage mouse embryos (after removal of the zona pellucida) to adhere rapidly and firmly upon a few minutes' exposure in vitro. Adhesion is successful at room temperature, and the cells remain agglutinated after transfer to room-temperature culture medium without PHA. The incubated aggregates continue their development. After surgical transfer to pseudopregnant recipients, aggregates comprising two different cellular genotypic populations can give rise to viable allophenic mice with both cellular genotypes. PHA treatment is thus a simple substitute for temperature-induced (37°C) aggregation of mouse blastomeres in vitro, and it provides a new and efficient tool for experimental studies of mammalian development.",

author = "Beatrice Mintz and Gearhart, {John D.} and Guymont, {Agnes O.}",

year = "1973",

doi = "10.1016/0012-1606(73)90331-X",

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T1 - Phytohemagglutinin-mediated blastomere aggregation and development of allophenic mice

AU - Mintz, Beatrice

AU - Gearhart, John D.

AU - Guymont, Agnes O.

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Y1 - 1973

N2 - Phytohemagglutinin (PHA) causes cells from two or more preimplantation-stage mouse embryos (after removal of the zona pellucida) to adhere rapidly and firmly upon a few minutes' exposure in vitro. Adhesion is successful at room temperature, and the cells remain agglutinated after transfer to room-temperature culture medium without PHA. The incubated aggregates continue their development. After surgical transfer to pseudopregnant recipients, aggregates comprising two different cellular genotypic populations can give rise to viable allophenic mice with both cellular genotypes. PHA treatment is thus a simple substitute for temperature-induced (37°C) aggregation of mouse blastomeres in vitro, and it provides a new and efficient tool for experimental studies of mammalian development.

AB - Phytohemagglutinin (PHA) causes cells from two or more preimplantation-stage mouse embryos (after removal of the zona pellucida) to adhere rapidly and firmly upon a few minutes' exposure in vitro. Adhesion is successful at room temperature, and the cells remain agglutinated after transfer to room-temperature culture medium without PHA. The incubated aggregates continue their development. After surgical transfer to pseudopregnant recipients, aggregates comprising two different cellular genotypic populations can give rise to viable allophenic mice with both cellular genotypes. PHA treatment is thus a simple substitute for temperature-induced (37°C) aggregation of mouse blastomeres in vitro, and it provides a new and efficient tool for experimental studies of mammalian development.

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Phytohemagglutinin-mediated blastomere aggregation and development of allophenic mice

Abstract

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