Phytohemagglutinin-mediated blastomere aggregation and development of allophenic mice

Beatrice Mintz, John D. Gearhart, Agnes O. Guymont

Research output: Contribution to journalArticlepeer-review

Abstract

Phytohemagglutinin (PHA) causes cells from two or more preimplantation-stage mouse embryos (after removal of the zona pellucida) to adhere rapidly and firmly upon a few minutes' exposure in vitro. Adhesion is successful at room temperature, and the cells remain agglutinated after transfer to room-temperature culture medium without PHA. The incubated aggregates continue their development. After surgical transfer to pseudopregnant recipients, aggregates comprising two different cellular genotypic populations can give rise to viable allophenic mice with both cellular genotypes. PHA treatment is thus a simple substitute for temperature-induced (37°C) aggregation of mouse blastomeres in vitro, and it provides a new and efficient tool for experimental studies of mammalian development.

Original languageEnglish (US)
Pages (from-to)195-199
Number of pages5
JournalDevelopmental Biology
Volume31
Issue number1
DOIs
StatePublished - 1973
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology

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