Physiology and morphology of complex spiking neurons in the guinea pig dorsal cochlear nucleus

P. B. Manis, G. A. Spirou, D. D. Wright, S. Paydar, David Kay Ryugo

Research output: Contribution to journalArticle

Abstract

Intracellular recordings from the dorsal cochlear nucleus have identified cells with both simple and complex action potential waveforms. We investigated the hypothesis that cartwheel cells are a specific cell type that generates complex action potentials, based on their analogous anatomical, developmental, and biochemical similarities to cerebellar Purkinje cells, which are known to discharge complex action potentials. Intracellular recordings were made from a brain slice preparation of the guinea pig dorsal cochlear nucleus. A subpopulation of cells discharged a series of two or three action potentials riding on a slow depolarization as an all-or-none event; this discharge pattern is called a complex spike or burst. These cells also exhibited anodal break bursts, anomalous rectification, subthreshold inward rectification, and frequent inhibitory postsynaptic potentials (IPSPs). Seven complex-spiking cells were stained with intracellular dyes and subsequently identified as cartwheel neurons. In contrast, six identified simple-spiking cells recorded in concurrent experiments were pyramidal cells. The cartwheel cell bodies reside in the lower part of layer 1 and the upper part of layer 2 of the nucleus. The cells are characterized by spiny dendrites penetrating the molecular layer, a lack of basal dendritic processes, and an axonal plexus invading layers 2 and 3, and the inner regions of layer 1. The cartwheel cell axons made putative synaptic contacts at the light microscopic level with pyramidal cells and small cells, including stellate cells, granule cells, and other cartwheel cells in layers 1 and 2. The axonal plexus of individual cartwheel cells suggests that they can inhibit cells receiving input from either the same or adjacent parallel fibers and that this inhibition is distributed along the isofrequency contours of the nucleus.

Original languageEnglish (US)
Pages (from-to)261-276
Number of pages16
JournalJournal of Comparative Neurology
Volume348
Issue number2
StatePublished - 1994

Fingerprint

Cochlear Nucleus
Guinea Pigs
Neurons
Action Potentials
Pyramidal Cells
Inhibitory Postsynaptic Potentials
Purkinje Cells
Dendrites

Keywords

  • auditory system
  • dye staining
  • electrophysiology
  • hearing
  • intracellular recording

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Physiology and morphology of complex spiking neurons in the guinea pig dorsal cochlear nucleus. / Manis, P. B.; Spirou, G. A.; Wright, D. D.; Paydar, S.; Ryugo, David Kay.

In: Journal of Comparative Neurology, Vol. 348, No. 2, 1994, p. 261-276.

Research output: Contribution to journalArticle

Manis, P. B. ; Spirou, G. A. ; Wright, D. D. ; Paydar, S. ; Ryugo, David Kay. / Physiology and morphology of complex spiking neurons in the guinea pig dorsal cochlear nucleus. In: Journal of Comparative Neurology. 1994 ; Vol. 348, No. 2. pp. 261-276.
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