Physiological regulation of the immunological synapse by agrin

A. A. Khan, C. Bose, L. S. Yam, Mark J Soloski, F. Rupp

Research output: Contribution to journalArticle

Abstract

T cell activation is dependent on both a primary signal delivered through the T cell receptor and a secondary costimulatory signal mediated by coreceptors. Although controversial costimulation is thought to act through the specific redistribution and clustering of membrane and intracellular kinase-rich lipid raft microdomains at the contact site between T cells and antigen-presenting cells. This site has been termed the immunological synapse. Endogenous mediators of raft clustering in lymphocytes have not been identified, although they are essential for T cell activation. We now demonstrate that agrin, an aggregating protein crucial for formation of the neuromuscular junction, is also expressed in lymphocytes and is important in reorganization of membrane lipid microdomains and setting the threshold for T cell signaling. Our data show that agrin induces the aggregation of signaling proteins and the creation of signaling domains in both immune and nervous systems through a common lipid raft pathway.

Original languageEnglish (US)
Pages (from-to)1681-1686
Number of pages6
JournalScience
Volume292
Issue number5522
DOIs
StatePublished - Jun 1 2001

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Agrin
Immunological Synapses
T-Lymphocytes
Cluster Analysis
Lymphocytes
Membrane Microdomains
Lipids
Intracellular Membranes
Neuromuscular Junction
Viral Tumor Antigens
Antigen-Presenting Cells
Membrane Lipids
T-Cell Antigen Receptor
Nervous System
Immune System
Proteins
Phosphotransferases

ASJC Scopus subject areas

  • General

Cite this

Physiological regulation of the immunological synapse by agrin. / Khan, A. A.; Bose, C.; Yam, L. S.; Soloski, Mark J; Rupp, F.

In: Science, Vol. 292, No. 5522, 01.06.2001, p. 1681-1686.

Research output: Contribution to journalArticle

Khan, AA, Bose, C, Yam, LS, Soloski, MJ & Rupp, F 2001, 'Physiological regulation of the immunological synapse by agrin', Science, vol. 292, no. 5522, pp. 1681-1686. https://doi.org/10.1126/science.1056594
Khan, A. A. ; Bose, C. ; Yam, L. S. ; Soloski, Mark J ; Rupp, F. / Physiological regulation of the immunological synapse by agrin. In: Science. 2001 ; Vol. 292, No. 5522. pp. 1681-1686.
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