TY - JOUR
T1 - Physiological glucose is critical for optimized neuronal viability and AMPK responsiveness in vitro
AU - Kleman, Amy M.
AU - Yuan, Jason Y.
AU - Aja, Susan
AU - Ronnett, Gabriele V.
AU - Landree, Leslie E.
N1 - Funding Information:
This work was funded by NIH grants from the NINDS, NIDCD, and NIDDK to G.V.R. We would like to thank FASgen, Inc. for providing C75 and V.H. Routh for helpful discussion at the onset of this project.
PY - 2008/1/30
Y1 - 2008/1/30
N2 - Understanding the mechanisms that govern neuronal responses to oxidative and metabolic stress is essential for therapeutic intervention. In vitro modeling is an important approach for these studies, as the metabolic environment influences neuronal responses. Surprisingly, most neuronal culture methods employ conditions that are non-physiological, especially with regards to glucose concentrations, which often exceed 20 mM. This concentration is a significant departure from physiological glucose levels, and even several-fold greater than that seen during severe hyperglycemia. The goal of this study was to establish a physiological neuronal culture system that will facilitate the study of neuronal energy metabolism and responses to metabolic stress. We demonstrate that the metabolic environment during preparation, plating, and maintenance of cultures affects neuronal viability and the response of neuronal pathways to changes in energy balance.
AB - Understanding the mechanisms that govern neuronal responses to oxidative and metabolic stress is essential for therapeutic intervention. In vitro modeling is an important approach for these studies, as the metabolic environment influences neuronal responses. Surprisingly, most neuronal culture methods employ conditions that are non-physiological, especially with regards to glucose concentrations, which often exceed 20 mM. This concentration is a significant departure from physiological glucose levels, and even several-fold greater than that seen during severe hyperglycemia. The goal of this study was to establish a physiological neuronal culture system that will facilitate the study of neuronal energy metabolism and responses to metabolic stress. We demonstrate that the metabolic environment during preparation, plating, and maintenance of cultures affects neuronal viability and the response of neuronal pathways to changes in energy balance.
KW - AICAR
KW - AMP-activated protein kinase
KW - ATP
KW - C75
KW - Neuronal culture
KW - Physiological glucose
UR - http://www.scopus.com/inward/record.url?scp=36849019542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36849019542&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2007.08.028
DO - 10.1016/j.jneumeth.2007.08.028
M3 - Article
C2 - 17936912
AN - SCOPUS:36849019542
SN - 0165-0270
VL - 167
SP - 292
EP - 301
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 2
ER -