TY - JOUR
T1 - Physical mapping of the human cytomegalovirus (HCMV) (Towne) DNA polymerase gene
T2 - DNA-mediated transfer of a genetic marker for an HCMV gene
AU - D'Aquila, Richard T.
AU - Hayward, Gary S.
AU - Summers, William C.
N1 - Funding Information:
We thank Wilma Summers for much helpful advice. Robert LaFem-ina, Marcia Lewis, Bill Sugden, Sandy Weller, and Don Coen provided clones. Abbott Laboratories provided PAA. This research was supported by the Yale Physician Scientist Program Award (U.S. Public Health Service Grant DK 01423).
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1989/7
Y1 - 1989/7
N2 - DNA-mediated transfer of a drug resistance marker (phosphonoacetate resistance for the HVMW (Towne) DNA polymerase (pol) gene has been used to genetically confirm the physical localization of the HCMV (Towne) DNA pol gene. The HCMV (Towne) genomic region homologous to the pol genes of other herpesviruses was first identified by moderate stringency Southern hybridization. Restriction fragments from this region were molecularly cloned from previously characterized phosphonoacetic acid resistant (PAAr)HCMV genomes (R. T. D'Aquila and W. C. Summers, J. Virol., 61. 1291-1295, 1987). A high frequency of recombinant PAAr virus was found among the progeny of cotransfections of infectious, wild-type HCMV (Towne) DNA only with pol-homologous restriction fragments cloned from PAAr HCMV. The co-transfection technique described here may facilitate further gene mapping in HCMV. The results presented here provide functional proof that the HCMV pol gene is encoded by the sequences previously identified as homologous to other herpesvirus pol genes.
AB - DNA-mediated transfer of a drug resistance marker (phosphonoacetate resistance for the HVMW (Towne) DNA polymerase (pol) gene has been used to genetically confirm the physical localization of the HCMV (Towne) DNA pol gene. The HCMV (Towne) genomic region homologous to the pol genes of other herpesviruses was first identified by moderate stringency Southern hybridization. Restriction fragments from this region were molecularly cloned from previously characterized phosphonoacetic acid resistant (PAAr)HCMV genomes (R. T. D'Aquila and W. C. Summers, J. Virol., 61. 1291-1295, 1987). A high frequency of recombinant PAAr virus was found among the progeny of cotransfections of infectious, wild-type HCMV (Towne) DNA only with pol-homologous restriction fragments cloned from PAAr HCMV. The co-transfection technique described here may facilitate further gene mapping in HCMV. The results presented here provide functional proof that the HCMV pol gene is encoded by the sequences previously identified as homologous to other herpesvirus pol genes.
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U2 - 10.1016/0042-6822(89)90546-1
DO - 10.1016/0042-6822(89)90546-1
M3 - Article
C2 - 2545037
AN - SCOPUS:0024324134
SN - 0042-6822
VL - 171
SP - 312
EP - 316
JO - Virology
JF - Virology
IS - 1
ER -