Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA

Alexander von Gabain, Gary S. Hayward, Hermann Bujard

Research output: Contribution to journalArticlepeer-review

Abstract

The DNA of bacteriophage T5+ (molecular weight 76×106 dalton) has been dissected by various specific endonucleases. The restriction enzymes HindIII and EcoRI produce 16 and 7 fragments respectively, whereas Sal and Sma produce 4 fragments each. Complete cleavage maps were established for the enzymes EcoRI, Sal and Sma and an almost complete map for HindIII. Furthermore the location and size of the deletions St 20, St 14, b3, St 0 and b1 were determined. The correlation of the genetic and functional map of the phage with the arrangement of fragments produced by the different enzymes has been established.

Original languageEnglish (US)
Pages (from-to)279-290
Number of pages12
JournalMGG Molecular & General Genetics
Volume143
Issue number3
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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