TY - JOUR
T1 - Physical mapping of chromosome 8p22 markers and their homozygous deletion in a metastatic prostate cancer
AU - Bova, G. Steven
AU - MacGrogan, Donal
AU - Levy, Alina
AU - Pin, Sokhom S.
AU - Bookstein, Robert
AU - Isaacs, William B.
N1 - Funding Information:
We thank Dr. Mitsuru Emi and Dr. Yoshiyuki Fujiwara for the gift of the MSR-32 probe, Dr. Y. Nakamura for cosmids cCI8-2644 and cCI8-1051, Dr. Dunne Fong for CTSB probe pCB1, Dr. Peter O'Connell for YAC clones, Dr. R. Leach for FISH analysis of 946_c_9, Dr. Ann Killary for somatic cell hybrid HA(8), and Dr. Michael Wagner for use of the Chromosome 8 mapping panel. This work was supported by NCI Grants CA58236, CA59457, CA60358, and CA55231.
PY - 1996/7/1
Y1 - 1996/7/1
N2 - Numerous studies have implicated the short arm of chromosome 8 as the site of one or more tumor suppressor genes inactivated in carcinogenesis of the prostate, colon, lung, and liver. Previously, we identified a homozygous deletion on chromosome 8p22 in a metastatic prostate cancer. To map this homozygous deletion physically, long-range restriction mapping was performed using yeast artificial chromosomes (YACs) spanning approximately 2 Mb of chromosome band 8p22. Subcloned genomic DNA and cDNA probes isolated by hybrid capture from these YACs were mapped in relation to one another, reinforcing map integrity. Mapped single-copy probes from the region were then applied to DNA isolated from a metastatic prostate cancer containing a chromosome 8p22 homozygous deletion and indicated that its deletion spans 730-970 kb. Candidate genes PRLTS (PDGF-receptor β-like tumor suppressor) and CTSB (cathepsin B) are located outside the region of homozygous deletion. Genethon marker D8S549 is located approximately at the center of this region of homozygous deletion. Two new microsatellite polymorphisms, D8S1991 and D8S1992, also located within the region of homozygous deletion on chromosome 8p22, are described. Physical mapping places cosmid CI8-2644 telomeric to MSR (macrophage scavenger receptor), the reverse of a previously published map, altering the interpretation of published deletion studies. This work should prove helpful in the identification of candidate tumor suppressor genes in this region.
AB - Numerous studies have implicated the short arm of chromosome 8 as the site of one or more tumor suppressor genes inactivated in carcinogenesis of the prostate, colon, lung, and liver. Previously, we identified a homozygous deletion on chromosome 8p22 in a metastatic prostate cancer. To map this homozygous deletion physically, long-range restriction mapping was performed using yeast artificial chromosomes (YACs) spanning approximately 2 Mb of chromosome band 8p22. Subcloned genomic DNA and cDNA probes isolated by hybrid capture from these YACs were mapped in relation to one another, reinforcing map integrity. Mapped single-copy probes from the region were then applied to DNA isolated from a metastatic prostate cancer containing a chromosome 8p22 homozygous deletion and indicated that its deletion spans 730-970 kb. Candidate genes PRLTS (PDGF-receptor β-like tumor suppressor) and CTSB (cathepsin B) are located outside the region of homozygous deletion. Genethon marker D8S549 is located approximately at the center of this region of homozygous deletion. Two new microsatellite polymorphisms, D8S1991 and D8S1992, also located within the region of homozygous deletion on chromosome 8p22, are described. Physical mapping places cosmid CI8-2644 telomeric to MSR (macrophage scavenger receptor), the reverse of a previously published map, altering the interpretation of published deletion studies. This work should prove helpful in the identification of candidate tumor suppressor genes in this region.
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U2 - 10.1006/geno.1996.0321
DO - 10.1006/geno.1996.0321
M3 - Article
C2 - 8661103
AN - SCOPUS:0030200892
SN - 0888-7543
VL - 35
SP - 46
EP - 54
JO - Genomics
JF - Genomics
IS - 1
ER -