Physical-chemical determinants of turn conformations in globular proteins

Timothy O. Street, Nicholas C. Fitzkee, Lauren L. Perskie, George D. Rose

Research output: Contribution to journalArticlepeer-review


Globular proteins are assemblies of α-helices and β-strands, interconnected by reverse turns and longer loops. Most short turns can be classified readily into a limited repertoire of discrete backbone conformations, but the physical-chemical determinants of these distinct conformational basins remain an open question. We investigated this question by exhaustive analysis of all backbone conformations accessible to short chain segments bracketed by either an α-helix or a β-strand (i.e., α-segment-α, β-segment-β, α-segment-β, and β-segment-α) in a nine-state model. We find that each of these four secondary structure environments imposes its own unique steric and hydrogen-bonding constraints on the intervening segment, resulting in a limited repertoire of conformations. In greater detail, an exhaustive set of conformations was generated for short backbone segments having reverse-turn chain topology and bracketed between elements of secondary structure. This set was filtered, and only clashfree, hydrogen-bond-satisfied conformers having reverse-turn topology were retained. The filtered set includes authentic turn conformations, observed in proteins of known structure, but little else. In particular, over 99% of the alternative conformations failed to satisfy at least one criterion and were excluded from the filtered set. Furthermore, almost all of the remaining alternative conformations have close tolerances that would be too tight to accommodate side chains longer than a single β-carbon. These results provide a molecular explanation for the observation that reverse turns between elements of regular secondary can be classified into a small number of discrete conformations. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1720-1727
Number of pages8
JournalProtein Science
Issue number8
StatePublished - Aug 2007


  • Computational analysis of protein structure
  • Protein structure/folding
  • Reverse turns

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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