Physical and comparative mapping of distal mouse chromosome 16

D. E. Cabin, J. W. McKee-Johnson, L. E. Matesic, T. Wiltshire, E. E. Rue, A. E. Mjaatvedt, Y. K. Huo, J. R. Korenberg, Roger H Reeves

Research output: Contribution to journalArticle

Abstract

Distal mouse Chromosome 16 (Chr. 16) includes a region of conserved linkage with human Chromosome 21 (Chr. 21). Mouse models of Down syndrome based on trisomy of distal Chr. 16 have several phenotypes similar to those seen in human patients and have proven useful for correlating dosage imbalance of specific genes with specific developmental anomalies. The degree to which such findings can be related to Down syndrome depends on how well the conserved synteny is maintained. Twenty-four genes have been mapped in both species and there are no discordancies, but the region could carry hundreds of genes. Comparative sequence represents the ultimate comparative map and will aid in identification of genes and their regulatory sequences. A physical map of the distal 4.5 Mb of Chr. 16 has been assembled as an essential step toward a map of sequence-ready templates. The map consists of 51 YACs and 15 BACs and includes 18 transcripts, 9 of which are mapped for the first time in mouse, and 3 of which are, for the first time, described in either species. YAC fragmentation was used to precisely localize the 49 markers on the map. Comparison of this physical map with 1 that of the corresponding region on Chr. 21 shows conservation not only of gene order but of size in the 3 Mb from Cbr1 to Ets2; distal to Ets2, the human map is expanded.

Original languageEnglish (US)
Pages (from-to)940-950
Number of pages11
JournalGenome Research
Volume8
Issue number9
StatePublished - 1998

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Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 21
Down Syndrome
Genes
Synteny
Gene Order
Human Chromosomes
Regulator Genes
Phenotype

ASJC Scopus subject areas

  • Genetics

Cite this

Cabin, D. E., McKee-Johnson, J. W., Matesic, L. E., Wiltshire, T., Rue, E. E., Mjaatvedt, A. E., ... Reeves, R. H. (1998). Physical and comparative mapping of distal mouse chromosome 16. Genome Research, 8(9), 940-950.

Physical and comparative mapping of distal mouse chromosome 16. / Cabin, D. E.; McKee-Johnson, J. W.; Matesic, L. E.; Wiltshire, T.; Rue, E. E.; Mjaatvedt, A. E.; Huo, Y. K.; Korenberg, J. R.; Reeves, Roger H.

In: Genome Research, Vol. 8, No. 9, 1998, p. 940-950.

Research output: Contribution to journalArticle

Cabin, DE, McKee-Johnson, JW, Matesic, LE, Wiltshire, T, Rue, EE, Mjaatvedt, AE, Huo, YK, Korenberg, JR & Reeves, RH 1998, 'Physical and comparative mapping of distal mouse chromosome 16', Genome Research, vol. 8, no. 9, pp. 940-950.
Cabin DE, McKee-Johnson JW, Matesic LE, Wiltshire T, Rue EE, Mjaatvedt AE et al. Physical and comparative mapping of distal mouse chromosome 16. Genome Research. 1998;8(9):940-950.
Cabin, D. E. ; McKee-Johnson, J. W. ; Matesic, L. E. ; Wiltshire, T. ; Rue, E. E. ; Mjaatvedt, A. E. ; Huo, Y. K. ; Korenberg, J. R. ; Reeves, Roger H. / Physical and comparative mapping of distal mouse chromosome 16. In: Genome Research. 1998 ; Vol. 8, No. 9. pp. 940-950.
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